| 四川5个山(绵)羊品种随机扩增多态DNA分析 |
| |
| 引用本文： | 朱金秋,李力,叶丰,李达旭,李雅雯,邹游,杨志荣.四川5个山(绵)羊品种随机扩增多态DNA分析[J].中国畜牧杂志,2005,41(3):9-13. |
| |
| 作者姓名： | 朱金秋 李力 叶丰 李达旭 李雅雯 邹游 杨志荣 |
| |
| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室,四川成都,610064 |
| |
| 基金项目： | 四川省畜牧业“十五”重点科研项目 |
| |
| 摘 要： | 本研究参阅文献挑选 80个随机引物 ,从中筛选出 3 0个重复性好的引物 ,对四川黑山羊、南江黄羊、北川白山羊、成都麻羊 4个山羊品种和藏绵羊进行RAPD分析 ,并进一步对 1 3只南江黄羊个体 ,1 8只黑山羊个体的基因组DNA进行PCR扩增。用Nei氏公式计算品种间的遗传距离 ,用UPGMA法构建树状聚类图。结果表明 :黑山羊与南江黄羊的遗传距离最小 ,亲缘关系较近 ;藏绵羊与各品种间的遗传距离都很大 ,亲缘关系远 ;RAPD技术可作为一种有效的分子标记用于山羊品种之间遗传亲缘关系的分析
|
| 关 键 词： | 畜牧、兽医科学基础学科 RAPD 遗传距离 亲缘关系 山羊 |
| 文章编号： | 0258-7033(2005)03-0009-05 |
| 修稿时间： | 2004年7月12日 |
Study on Random Amplified Polymorphism DNA of Five Goat (Sheep) Breeds in Sichuan |
| |
| ZHU Jin-qiu,LI Li,YE Feng,LI Ya-wen,YANG Zhi-rong.Study on Random Amplified Polymorphism DNA of Five Goat (Sheep) Breeds in Sichuan[J].Chinese Journal of Animal Science,2005,41(3):9-13. |
| |
| Authors: | ZHU Jin-qiu LI Li YE Feng LI Ya-wen YANG Zhi-rong |
| |
| Institution: | ZHU Jin-qiu,LI Li,YE Feng,LI Ya-wen,YANG Zhi-rong~* |
| |
| Abstract: | In this study, DNA of goat(sheep)breeds, including Black goat, Nanjiang Brown goat, Beichuan white goat, Chengdu Ma goat and Xizang sheep were amplified with 30 random primers selected from 80 primers which have been used in RAPD analysis for goats and sheep. The individuals of 18 Black goats and 13 Nanjiang Brown goats were selected for further amplification. Genetic distance indexes among breeds were calculated by Nei, and the phylogenetic tree was constructed by UPGMA method. The results showed: The genetic distance between Black goat and Nanjiang Brown goat was the smallest, therefore Black goat was closely related to Nanjiang Brown goat. While the genetic distance between Xizang sheep and any other goat breeds were not closely related, so Xizang sheep was far related to any other goat breeds. The results indicated that RAPD was an effective marker for the analysis of genetic relationship among goat breeds. |
| |
| Keywords: | basal disciplines of animal and veterinary science RAPD genetic distance phylogenetic relationship goat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
