The effects of temperature on the uptake and metabolism of benzo[a]pyrene in isolated gill cells of the gulf toadfish,Opsanus beta |
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| Authors: | C J Kennedy P J Walsh |
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| Institution: | (1) Department of Biological Sciences, Simon Fraser University, Burnaby, B.C., Canada, V5A 1S6;(2) Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Science, University of Miami, Miami, Florida, U.S.A., 33149-1098 |
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| Abstract: | The effects of acclimation temperature and acute temperature change on the uptake and metabolism of the procarcinogen benzoa]pyrene
(BaP) by gill cells of the gulf toadfish, Opsanus beta, were examined. BaP was rapidly accumulated by isolated gill cells and uptake rates were directly proportional to BaP concentration
in the medium (1 to 100 μg/ml). Uptake rates were higher in cells isolated from fish acclimated to 18°C when compared to cells
from 28°C acclimated fish at all incubation temperatures. When cells were exposed to BaP at the respective acclimation temperatures
of the fish, uptake rates were similar (0.14 ± 0.01 at 18°C and 0.12 ± 0.01 μg BaP/s/10 mg cells at 28°C). This finding is
discussed in view of results which showed a partial compensation of membrane fluidity in plasma membranes isolated from fish
from the two acclimation temperatures. At higher incubation temperatures, cells from fish acclimated to 18°C metabolized BaP
at a greater rate than those at 28°C (49.6 ± 1.92 and 43.0 ± 2.24 μg/g/8h, respectively, at 23°C). Low but detectable activities
of common biotransformation enzymes (aryl hydrocarbon hydroxylase, glutathione-S-transferase) and cytochrome P-450 content
were found, however, no significant differences were evident between cells from fish acclimated to different temperatures.
To whom to address correspondence |
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| Keywords: | xenobiotics uptake metabolism benzo[a]pyrene polycyclic aromatic hydrocarbons gills toadfish Opsanus beta |
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