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Isolation and characterization of a regulator of G protein signaling (RGS16) gene homologue in yellow grouper Epinephelus awoara |
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| Authors: | Li Wang Xinzhong Wu |
| Institution: | aLaboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China |
| Abstract: | Regulators of G protein signaling (RGS) are GTPase-activating proteins (GAP) which act as modulators of G protein-coupled receptors. We isolated a RGS16 homologue in yellow grouper (Epinephelus awoara) spleen using suppression subtractive hybridization and RACE-PCR. The nucleotide sequence of yellow grouper RGS16 full-length cDNA was 700 bp and contained an open reading frame of 537 bp, encoding a putative protein of 178 amino acids. The encoded protein shows 47–61% identities to other homologues. RT-PCR analysis demonstrated that RGS16 was expressed in yellow grouper spleen and up-regulated in kidney heart, liver, and anterior kidney by lipopolysaccharide. This study will help towards validating the specific function of RGS in marine fish. |
| Keywords: | Regulator of G protein signalling 16 (RGS16) Suppression subtractive hybridization (SSH) Gene expression Epinephelus awoara |
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