|
|||||
|
|
| TGEV的sM、M和N基因克隆及特征分析 | |
| 引用本文： | 程杰,柳纪省,吴润,殷相平,李宝玉,兰喜,王辉.TGEV的sM、M和N基因克隆及特征分析[J].畜牧兽医学报,2005,36(7):695-700. |
| 作者姓名： | 程杰 柳纪省 吴润 殷相平 李宝玉 兰喜 王辉 |
| 作者单位： | 1. 中国农业科学院兰州兽医研究所,兰州,730046;兰州大学,兰州,730000 2. 中国农业科学院兰州兽医研究所,兰州,730046 3. 甘肃农业大学动物医学院,兰州,730070 |
| 基金项目： | 重大基础研究“973”前期专项(2004CCA00500) |
| 摘 要： | 参照(3enBank中收录的TGEV sM、M和N基因序列各设计1对特异引物，经RT-PCR扩增获得了TS株的相应基因片段，分别约为346、932和1217bp，其大小与预计的目的片段相符。与其他毒株的相应基因相比较，并经剪接后，TS株的sM、M和N基因全长分别为248、789和1149bp，各编码82个、262个和382个氨基酸；TS株与Purdue株、TF1株和96-1933株的sM基因核苷酸序列同源性分别为95．2％、92．7％和90．2％；推导氨基酸的同源性分别为95．9％、97．2％、98．8％；与Purdue株、TFl株、TGEVH株和96-1933株的M基因核苷酸序列同源性分别为95．2％、98．0％、99．6％和95．0％；推导氨基酸的同源性分别为97．0％、97．3％、98．5％、93．5％；与Purdue株、TF1株、FS722／70株、Korea株、TO14株、TC；EVH株和96-1933株的N基因核苷酸序列同源性分别为98．1％、97．7％、99．0％、98．3％、99．2％、99．0％和95．9％，推导氨基酸的同源性分别为97．9％、98．4％、99．0％、97．7％、99．5％、96．2％、96．6％。并对TS株基因间的保守序列和sM、M和N基因及其编码的相应氨基酸的结构特征进行了分析，发现sM和N基因在TGEV中保守；并提示在我国不仅存在有2个不同亚基因型的TGEV，而且我国的TGEV可能是输入性的。 |
| 关 键 词： | 猪传染性胃肠炎病毒 TGEV TS株 sM基因 M基因 N基因 克隆 |
| 文章编号： | 0366-6964(2005)07-0695-06 |
Cloning and Structural Characterization Analysis on the sM ,M and N Genes of Porcine Transmissible Gastroenteritis Virus |
|
| CHENG Jie,LIU Ji-xing,WU Run,YIN Xiang-ping,LI Bao-yu,LAN Xi,WANG Hui.Cloning and Structural Characterization Analysis on the sM ,M and N Genes of Porcine Transmissible Gastroenteritis Virus[J].Acta Veterinaria et Zootechnica Sinica,2005,36(7):695-700. | |
| Authors: | CHENG Jie LIU Ji-xing WU Run YIN Xiang-ping LI Bao-yu LAN Xi WANG Hui |
| Abstract: | Three structure protein genes of strain TS of transmissible gastroenteritis virus(TGEV), sM(small membrane protein),M(integral membrane protein) and N(nucleocapsid protein) genes were obtained by RT-PCR using primers designed according to their published nucleotide sequence in GenBank, and they are 346 bp,932 bp,1 217 bp, respectively. After splicing, the respective lengths of the sM, M and N genes of strain TS of TGEV is 248 bp,789 bp and 1 149 bp and their predicted corresponding protein has 82, 262 and 382 amino acids(aa), respectively. The homology at the nucleotide levels for the sM gene between strain TS and Purdue,TFI,96-1933 was found to be 95.2%,92.7%,90.2% respectively, and that of the deduced aa sequence was 95.9%,97.2%,98.8% correspondingly. The nucleotide sequence homology of M gene of strains TS shared 95.2%,98.0%,99.6%,95.0% nucleotide identity and 97.0%,97.3%,98.5%,93.5% deduced amino acid with that of strains Purdue, TFI,TGEV H,96-1933. The nucleotide sequence homology of N gene between strain TS and Purdue, TFI, FS722/70, Korea,TO14,TGEV H, 96-1933 is 98.1%,97.7%,99.0%,98.3%,99.2%,99.0%,95.9% and putative amino acid homology is 97.9%,98.4%,99.0%,97.7%,99.5%,96.2%,96.6%, respectively. Sequence analysis indicated that sM and N genotypes of TGEV are conservative, there are two kinds sub-genes of TGEV in China and their origins are probably from abroad. |
| Keywords: | TGEV TS strain sM gene M gene N gene cloning |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |