| 水生生物近缘种和产地的分子生物学判别 |
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| 引用本文： | 孙超,苏彦平,刘洪波,杨健.水生生物近缘种和产地的分子生物学判别[J].鲑鳟渔业,2011(3):53-59. |
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| 作者姓名： | 孙超 苏彦平 刘洪波 杨健 |
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| 作者单位： | [1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,中国水产科学研究院内陆渔业生态环境与资源重点开放实验室,江苏无锡214081 |
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| 基金项目： | 国家自然科学基金（31072214）; 中央级公益性科研院所基本科研业务费专项资金项目（2011JBFA14） |
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| 摘 要： | 随着分子生物学技术的发展,渔业生物近缘种及产地的判别由形态学深入到了蛋白质和DNA水平。本文综述了目前已经应用于水生生物的相关分子生物学技术（包括色谱、免疫学判别以及蛋白的电泳、DNA的序列分析、PCR-RFLP、RAPD、AFLP、SNP、SSRI、SSR、Real-Time PCRs、pecies-specific primer PCR和PCRlab-on-a-chip等）以及它们各自的优缺点;在把握了这些研究进展的基础上,展望了相关技术的发展趋势。
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| 关 键 词： | 分子生物学 水生生物 近缘种 产地 判别 |
The Identification of Closely Related Species and Origin of Aquatic Organisms by Molecular Approaches |
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| Authors: | SUN Chao SU Yan-ping LIU Hong-bo YANG Jian |
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| Institution: | 1.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;2.Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China) |
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| Abstract: | With the development of molecular biology,the approaches for closely related species and origin identification of aquatic organisms has developed from morphological way to molecular level.The article summarizes current protein and DNA techniques that have been applied in corresponding identification（e.g.,chromatography,immunology identification,and protein electrophoresis,DNA sequence analysis,PCR-RFLP,RAPD,AFLP,SNP,SSR,ISSR,Real-Time PCR,species-specific primer PCR,PCR lab-on-a-chip） and makes comparison of their advantages and disadvantages.On the basis of the advances above,the future development tendency of the approaches is expected in the present article. |
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| Keywords: | molecular biology aquatic organism closely related species origin identification |
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