Hepatic RNA:DNA ratio as a reliable indicator of the growth performance of juvenile <Emphasis Type="Italic">Pelodiscus sinensis</Emphasis> under nutritional stress |
| |
| Authors: | Fangshuo Ji Quansen Xie Haiyan Liu Yujuan Li Zhencai Yang |
| |
| Institution: | 1.College of Life Science,Hebei Normal University,Shijiazhuang,China |
| |
| Abstract: | We investigated the reliability of the ratio of RNA to DNA in juvenile Pelodiscus sinensis under moderate nutritional stress from lack of sufficient protein. The control diet contained 45% protein. Two experimental diets containing 40 and 35% protein were used to trigger nutritional stress. One hundred fifty-six turtles were randomly divided into the three diet groups and fed for 90 days. Four turtles were randomly sampled from each group on days 2, 4, 7, 15, 30, 45, 60, 75 and 90 of the trial and their hepatic RNA:DNA was measured. Each turtle was weighed on days 15, 30, 45, 60, 75 and 90. Obvious positive correlations between RNA:DNA and specific growth rate (SGR) were found, which were affected by dietary protein levels. The exponential regression model of the relationship between RNA:DNA (x) and SGR (y) in turtles fed 45% protein diet was y = 0.8001e0.3072x , in the 40% protein diet it was y = 0.5374e0.4297x , and in the 35% protein diet it was y = 0.7867e0.369x . From day 7 until the end of the trial, RNA:DNA accurately distinguished differences among the three groups. We suggest that hepatic RNA:DNA is a sensitive and quick indicator of growth performance in juvenile turtles, especially in studies related to nutritional stress. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
