| 硫酸化20（S）-人参皂苷Rh2体外对鸡免疫活性细胞功能的影响 |
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| 引用本文： | 王鲁,付本懂,伊鹏霏,韦旭斌.硫酸化20（S）-人参皂苷Rh2体外对鸡免疫活性细胞功能的影响[J].贵州畜牧兽医,2009,33(2):10-12. |
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| 作者姓名： | 王鲁 付本懂 伊鹏霏 韦旭斌 |
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| 作者单位： | 1. 贵州大学动物科学学院,贵州,贵阳,550025
2. 吉林大学畜牧兽医学院,吉林,长春,130062 |
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| 基金项目： | 国家自然科学基金，贵州大学引进人才科研项目 |
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| 摘 要： | 为了评价硫酸化人参皂苷Rh2化学修饰前后免疫活性变化，采用MTT法及流式细胞术研究体外对ConA促鸡外周血淋巴细胞增殖的影响，采用乳酸脱氢酶释放法研究体外对鸡外周血NK细胞杀伤活性的影响。结果表明：Rh2、Rh2溶剂及硫酸化Rh2低浓度时都能促进淋巴细胞增殖，高浓度时能抑制淋巴细胞增殖，Rh2溶剂及硫酸化Rh2都显著（P〈0．05）或极显著（P〈0．01）高于同浓度的Rh2。Rh2及Rh2溶剂都能显著促进鸡外周血NK细胞杀伤活性，但Rh2极显著（P〈0．01）低于同浓度Rh2溶剂，而硫酸化Rh2表现一定促进作用。结果提示：Rb2体外能抑制免疫活性，化学修饰后的硫酸化Rh，体外能促进免疫活性。
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| 关 键 词： | 硫酸化Rh2 人参皂苷Rh2 淋巴细胞 NK细胞 |
Effect of Sulfated 20(S)-ginsenoside Rh_2 on Function of Chicken Immunocompetent Cells in Vitro |
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| Wang Lu,Fu Bendong,Yi Pengfei,Wei Xubin.Effect of Sulfated 20(S)-ginsenoside Rh_2 on Function of Chicken Immunocompetent Cells in Vitro[J].Guizhou Animal Science and Veterinary Medicine,2009,33(2):10-12. |
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| Authors: | Wang Lu Fu Bendong Yi Pengfei Wei Xubin |
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| Institution: | 1.College of Animal Science;Guizhou University;Guizhou Guiyang 550025;China;2.College of Animal Science and Veterinary Medicine;Jilin University;Jilin Changchun 130062;China |
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| Abstract: | To evaluate the effects of both 20(S)-ginsenoside-Rh2(Rh2) and sulfated of Rh2(S-Rh2) on the function of immunologic cells in vitro,the chicken peripheral lymphocyte proliferation was determined by MTT assay and flow cytometry,and the killing activity of NK cell of chicken peripheral blood was measured by lactate dehydrogenase releasing assay.The results showed that Rh2,solution adjuvant(dissolve Rh2 in RPMI-1640) and S-Rh2 had marked effect to improve the proliferation of lymphocytes at lower concentration... |
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| Keywords: | Sulfated of Rh2 Rh2 Lymphocyte Natural Killer Lymphocyte |
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