| tva受体基因起始密码子突变对鸡感染A亚群禽白血病病毒的影响 |
| |
| 引用本文： | 陈伟国,邝智祥,张翔宇,李文雪,徐慧娟,朱学凤,谢青梅.tva受体基因起始密码子突变对鸡感染A亚群禽白血病病毒的影响[J].华南农业大学学报,2023,44(3):367-373. |
| |
| 作者姓名： | 陈伟国 邝智祥 张翔宇 李文雪 徐慧娟 朱学凤 谢青梅 |
| |
| 作者单位： | 华南农业大学 动物科学学院/广东省畜禽健康养殖与环境控制重点实验室, 广东 广州 510642;广东爱健康生物科技有限公司, 广东 清远 511800 |
| |
| 基金项目： | 广东省重点领域研发计划(2020B020222001)；国家重点研发计划(2021YFD1300100)；清远市科技计划项目(2021DZX019) |
| |
| 摘 要： | 【目的】探索tva受体基因起始密码子突变(tva c.3G>A)对鸡感染A亚群禽白血病病毒(Avian leukemia virus subgroup A, ALV-A)的影响。【方法】利用Sanger测序和RT-PCR验证我国黄羽肉鸡存在tva c.3G>A突变。利用流式细胞术检测tva c.3G>A突变对鸡体外感染RCASBP(A)-GFP荧光报告病毒的影响。通过ALV-A体内攻毒试验，探究tva c.3G>A突变对鸡体内感染ALV-A的影响。利用直接测序方法对我国黄羽肉鸡品系tva c.3G>A突变位点进行基因分型。【结果】Sanger测序和RT-PCR结果鉴定我国黄羽肉鸡品系tva基因编码区第3位碱基由G突变为A，该突变引起tva基因起始密码子序列由ATG突变为ATA。流式细胞术检测结果显示野生型tva c.3G/G鸡胚成纤维细胞(Chicken embryo fibroblast, CEF)对RCASBP(A)-GFP易感，纯合突变型tva c.3A/A CEF抗RCASBP(A)-GFP感染，表明tva c.3G>A突变导致鸡体外抗RCA...
|
| 关 键 词： | A亚群禽白血病病毒 tva受体基因 起始密码子 突变 遗传抗性 黄羽肉鸡 |
| 收稿时间： | 2022/6/15 0:00:00 |
Effect of initiation codon mutation within tva receptor gene on chicken resistance to infection by avian leukemia virus subgroup A |
| |
| CHEN Weiguo,KUANG Zhixiang,ZHANG Xiangyu,LI Wenxue,XU Huijuan,ZHU Xuefeng,XIE Qingmei.Effect of initiation codon mutation within tva receptor gene on chicken resistance to infection by avian leukemia virus subgroup A[J].Journal of South China Agricultural University,2023,44(3):367-373. |
| |
| Authors: | CHEN Weiguo KUANG Zhixiang ZHANG Xiangyu LI Wenxue XU Huijuan ZHU Xuefeng XIE Qingmei |
| |
| Institution: | College of Animal Science, South China Agricultural University/Guangdong Provincial Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou 510642, China;Guangdong Love-Health Biotechnology Co., Ltd., Qingyuan 511800, China |
| |
| Abstract: | Objective To explore the effect of initiation codon mutation within tva receptor gene (tva c.3G>A) on resistance of chickens to infection by avian leukemia virus subgroup A (ALV-A).Method Sanger sequencing and RT-PCR were used to verify the presence of tva c.3G>A mutation in Chinese yellow-feathered broilers. The effect of tva c.3G>A mutation on infection of chickens by RCASBP(A)-GFP fluorescence reporter virus in vitro was detected using flow cytometry. The effect of tva c.3G>A mutation on infection of chickens by ALV-A was investigated using ALV-A challenge test in vivo. Direct sequencing was used to genotype tva c.3G>A mutation site within Chinese yellow-feathered broiler lines.Result The results of Sanger sequencing and RT-PCR identified the mutation from G to A on the third base in the coding region of tva gene of Chinese yellow-feathered broilers, which caused the mutation from ATG to ATA in the initial codon sequence of tva gene. The result of flow cytometry showed that chicken embryo fibroblasts (CEFs) of wild-type tva c.3G/G were susceptible to infection by RCASBP(A)-GFP, while the homozygous mutant tva c.3A/A CEFs were resistant to infection by RCASBP(A)-GFP, indicating that tva c.3G>A mutation led to chicken resistance to infection by RCASBP(A)-EGFP in vitro. The results of ALV-A challenge test in vivo also indicated that tva c.3G>A mutation led to chicken resistance to infection by ALV-A. Genotyping of tva c.3G>A revealed that homozygous resistance genotype tva c.3A/A was present in lines CB01, CB08, CB10 and CB15, with the frequencies of 0.10, 0.15, 0.23 and 0.08, respectively.Conclusion The tva c.3G>A mutation causes chicken resistance to infection by ALV-A in vitro and in vivo, and the tva c.3G>A mutation site can be used as the genetic resistance site of ALV-A. |
| |
| Keywords: | Avian leukemia virus subgroup A tva receptor gene Initiation codon Mutation Genetic resistance Yellow-feathered broiler |
|
| 点击此处可从《华南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华南农业大学学报》下载免费的PDF全文 |
