Biological activity of recombinant bovine interferon tau using an Autographa californica nuclear polyhedrosis virus expression system |
| |
| Authors: | Takahashi Hitomi Inumaru Shigeki Takahashi Masashi Watanabe Satoko Iga Kosuke Yokomizo Yuichi Geshi Masaya Okano Akira Okuda Kiyoshi |
| |
| Institution: | Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan. orca@affrc.go.jp |
| |
| Abstract: | Bovine interferon (bIFN) tau, which plays a key role in maternal-fetal recognition of pregnancy, was expressed by an Autographa californica nuclear polyhedrosis virus expression system. cDNA coding bIFNtau was derived from cultured trophoblast cells. The recombinant (r) bIFNtau had high antiviral activity (1 x 10 (8) IU/mg) and the molecular weight of rbIFNtau was estimated to be 23 kDa by Western blotting analysis. We investigated the biological effect of rbIFNtau on prostaglandin (PG) F(2alpha) synthesis in cultured bovine endometrial epithelial cells in the presence or absence of oxytocin (OT, 100 nM). rbIFNtau suppressed basal and OT-induced PGF(2alpha) production in a dose-dependent manner (1-1,000 ng/ml). These results showed that biologically active rbIFNtau was produced in the baculovirus expression system, and that rbIFNtau had the ability to suppress the synthesis of PGF(2alpha) from bovine endometrial epithelial cells. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
