| 牛乳中超氧化物歧化酶(SOD)的提纯与鉴定 |
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| 引用本文： | 孙庆林,常梅秀,周书勤,姜广军.牛乳中超氧化物歧化酶(SOD)的提纯与鉴定[J].内蒙古农业大学学报(自然科学版),1998(4). |
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| 作者姓名： | 孙庆林 常梅秀 周书勤 姜广军 |
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| 作者单位： | 内蒙古农牧学院生物工程系，包头市第六中学，内蒙古农牧学院食品工程系 |
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| 摘 要： | 新鲜牛乳经超速离心，氯仿乙醇处理，丙酮沉淀、透析、ＤＥＡＥ纤维素柱层析等步骤使其中Ｃｕ－ＺｎＳＯＤ得到了纯化，提纯后酶的比活为１４９．５Ｖ／ｍｇ。该酶对Ｈ２Ｏ２敏感，并且在２７２ｎｍ和６７５ｎｍ处各有一吸收峰，证明被纯化的酶是Ｃｕ－ＺｎＳＯＤ。
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| 关 键 词： | 牛乳 超氧化物歧化酶 纯化 |
PURIFICATION AND IDENTIFICATION OF SUPEROXIDE DISMVTASE FROM MILK |
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| Sun Qinglin Chang Meixiu,Zhou Shuqin Jiang Guangjun.PURIFICATION AND IDENTIFICATION OF SUPEROXIDE DISMVTASE FROM MILK[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),1998(4). |
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| Authors: | Sun Qinglin Chang Meixiu Zhou Shuqin Jiang Guangjun |
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| Abstract: | A copper/zinc superoxide dismutase from fresh milk was purified to homogeneity by ultracentrifugztion. Chloroform ethanol treatment. acetone sedimention, dialyzation, DEAE-cellulose chromatography. the specific activity of the purified enzyme is 149.5V/mg. The enzyme is sensitire to H 2 O 2 .The enzyme enhibits maximam absortion at 272nm and 675nm. This facts have proved that the enzyme is copper/zinc SOD. |
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| Keywords: | milk SOD Purification |
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