| 鸡、鸽、虎呼吸道和消化道黏膜上皮细胞表面流感病毒受体类型的检测 |
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| 引用本文： | 马明,刘月焕,陈明勇,韩春华,林健.鸡、鸽、虎呼吸道和消化道黏膜上皮细胞表面流感病毒受体类型的检测[J].中国农业科学,2009,42(8):2958-2965. |
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| 作者姓名： | 马明 刘月焕 陈明勇 韩春华 林健 |
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| 作者单位： | 1. 中国农业大学动物医学院,北京,100193
2. 北京市农林科学院畜牧兽医研究所,北京,100097 |
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| 基金项目： | 北京市自然科学基金，国家科学基金 |
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| 摘 要： | 【目的】检测鸡、鸽和虎体内的流感病毒受体的类型和分布,以期解释3种动物对禽流感病毒易感性差异的机制。【方法】使用地高辛标记的外源性凝集素染色方法检测这些动物的喉头、气管、肺脏和肠道（直肠）的上皮细胞表面SAα2,6Gal和SAα2,3Gal连接键的类型。【结果】SPF鸡的上呼吸道黏膜上皮细胞表面有大量的SAα2,3Gal和少量的SAα2,6Gal;肺房上皮细胞表面只有SAα2,3Gal;而直肠黏膜上皮中SAα2,6Gal和SAα2,3Gal都没有表达。成年鸽的上呼吸道黏膜上皮细胞表面只有SAα2,6Gal,而没有SAα2,3Gal;而下呼吸道中SAα2,6Gal和SAα2,3Gal都没有;直肠黏膜上皮细胞只有SAα2,3Gal。虎的呼吸道和消化道（直肠）黏膜上皮细胞表面有大量的SAα2,6Gal和SAα2,3Gal。【结论】鸡和虎具有禽流感病毒受体,对禽流感病毒易感,鸽不具备禽流感病毒受体,因此,鸽对禽流感病毒不易感。
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| 关 键 词： | 流感病毒受体 唾液酸 鸡 鸽 虎 |
| 收稿时间： | 2008-10-07; |
Influenza Virus Receptor Detection on Chicken,Pigeon and Tiger Respiratory and Intestine Tract Epithelium Cells |
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| MA Ming,LIU Yue-huan,CHEN Ming-yong,HAN Chun-hua,LIN Jian.Influenza Virus Receptor Detection on Chicken,Pigeon and Tiger Respiratory and Intestine Tract Epithelium Cells[J].Scientia Agricultura Sinica,2009,42(8):2958-2965. |
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| Authors: | MA Ming LIU Yue-huan CHEN Ming-yong HAN Chun-hua LIN Jian |
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| Institution: | (Colloge of Veterinary Medicine, China Agricultural University)
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| Abstract: | 【Objective】 To detect the type and distribution of influenza virus receptors in chicken, pigeon and tiger, then find the mechanism to explain the difference of these animals’ affectability to avian influenza virus. 【Method】 The type of SAα2, 6Gal and SAα2, 3Gal linkage on these animals larynx, trachea, lung and intestine (colon) epithelium cells was detected by digoxigenin-labeled lectin staining. 【Result】 Abundant SAα2, 3Gal and slight SAα2, 6Gal on specific-pathogen free chickens upper respiratory tract epithelium cells were observed, the alveolar epithelium cells only express SAα2, 3Gal, however the colon epithelium cells express neither SAα2, 6Gal nor SAα2, 3Gal. Adult pigeon upper respiratory tract epithelium cells only express SAα2, 6Gal, but not SAα2, 3Gal. Pigeon lower respiratory tract express neither SAα2, 6Gal nor SAα2, 3Gal.Pigeon colon epithelium cells only express SAα2, 3Gal. There are abundant SAα2, 6Gal and SAα2, 3Gal on tiger respiratory and intestine tract epithelium cells. 【Conclusion】 These data have provided a rational explanation for why chickens and tigers are susceptible to avian influenza virus, while pigeons are not. |
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| Keywords: | influenza virus receptor sialic acid chicken pigeon tiger |
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