| 犬弓首蛔虫abcg-5基因的克隆及序列分析 |
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| 引用本文： | 黄汉成,江艾耘,罗永莉,杨晓迪,李相兰,胡志刚,周荣琼.犬弓首蛔虫abcg-5基因的克隆及序列分析[J].西南农业大学学报,2019,41(3):23-29. |
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| 作者姓名： | 黄汉成 江艾耘 罗永莉 杨晓迪 李相兰 胡志刚 周荣琼 |
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| 作者单位： | 西南大学动物科学学院 |
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| 基金项目： | 国家自然科学基金项目（31672541；31172313）；中央高校基本科研业务项目（XDJK2016E087；XDJK2017D080）. |
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| 摘 要： | 研究犬弓首蛔虫(Toxocara canis,T.canis)腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporters, ABC)亚家族G5基因(abcg-5)的分子特征.根据犬弓首蛔虫基因组数据库中的Tc-abcg-5基因序列设计引物,通过PCR技术克隆Tc-abcg-5全长基因,并进行多重序列比对和种系发育进化树分析.结果表明:该基因全长为1 902 bp,编码633个氨基酸.功能结构域分析发现TcABCG5蛋白包含1个ABC转运蛋白结构域和6个跨膜区,同时发现了高度保守的Walker A和Walker B模体. GO分析显示ABCG5具有ATP结合和ATP酶活性.种系发育进化树分析显示TcABCG5与猪蛔虫进化关系较近,与哺乳动物进化关系较远.
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| 关 键 词： | 犬弓首蛔虫 abcg-5基因 克隆 序列分析 |
| 收稿时间： | 2018/3/19 0:00:00 |
Cloning and Sequence Analysis of the Full-Length abcg-5 Gene of Toxocara canis |
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| HUANG Han-cheng,JIANG Ai-yun,LUO Yong-li,YANG Xiao-di,LI Xiang-lan,HU Zhi-gang,ZHOU Rong-qiong.Cloning and Sequence Analysis of the Full-Length abcg-5 Gene of Toxocara canis[J].Journal of Southwest Agricultural University,2019,41(3):23-29. |
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| Authors: | HUANG Han-cheng JIANG Ai-yun LUO Yong-li YANG Xiao-di LI Xiang-lan HU Zhi-gang ZHOU Rong-qiong |
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| Institution: | College of Animal Science, Southwest University, Rongchang Chongqing 402460, China |
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| Abstract: | To study the molecular characteristics of ATP-binding cassette sub-family G member 5 (abcg-5) in Toxocara canis, the full-length coding sequence of Tc-abcg-5 gene was amplified using PCR amplification, multiple sequence alignment (MSA) was made and a phylogenetic tree was constructed. The results showed that the entire sequence of Tc-abcg-5 gene contained 1 902 bp, encoding a protein of 633 amino acids. TcABCG5 was found to contain one ABC transporter domain and six transmembrane regions. The highly conserved Walker A and Walker B motifs of the ABCG sub-family were also found. Gene ontology (GO) annotations showed that TcABCG5 had the molecular function of ATP binding and ATPase activity. Phylogenetic analysis indicated that TcABCG5 shared a high level of amino-acid sequence similarity with Ascaris suum, and shared a low level of amino-acid sequence similarity with mammals. This study is expected to lay a foundation for further functional researches of TcABCG5. |
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| Keywords: | Toxocara canis ATP-binding cassette subfamily G member 5 (abcg-5) gene cloning sequence analysis |
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