| 侵染广东番茄的番茄褪绿病毒分子鉴定 |
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| 引用本文： | 汤亚飞,何自福,佘小漫,蓝国兵.侵染广东番茄的番茄褪绿病毒分子鉴定[J].植物保护,2017,43(2):133-137. |
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| 作者姓名： | 汤亚飞 何自福 佘小漫 蓝国兵 |
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| 作者单位： | 1. 广东省农业科学院植物保护研究所,广州510640;广东省植物保护新技术重点实验室,广州510640;2. 广东省农业科学院植物保护研究所,广州,510640 |
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| 基金项目： | 公益性行业(农业)科研专项(201303028); 广东省科技计划项目(2014B070706017, 2015A020209070) |
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| 摘 要： | 在广东番茄上发现一种新病害,病株表现为叶片褪绿,叶脉颜色变深及叶片增厚等症状。利用番茄褪绿病毒Tomato chlorosis virus(ToCV)HSP70基因的两对特异引物对番茄病样进行RT-PCR检测,结果表明,从所采集的6份病样中均扩增到预期大小的DNA特异片段。对其中1份样品的扩增片段进行克隆与序列分析,结果表明,扩增片段包括1个长度为1 665个核苷酸的完整病毒基因,其核苷酸序列与已报道的ToCV HSP70基因有较高的同源性,表明广东番茄受到了ToCV的侵染。但ToCV广东番茄分离物的HSP70序列与国内外已报道的各分离物的同源性均低于82%,存在较大差异,其中与塞浦路斯tomato、约旦JU_20分离物的同源性最高,为81.8%。这是ToCV在广东发生的首次报道,也是该病毒HSP70基因序列存在显著差异分离物的首次发现。
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| 关 键 词： | 番茄病毒病 番茄褪绿病毒 HSP70基因 |
| 收稿时间： | 2016/4/20 0:00:00 |
| 修稿时间： | 2016/6/27 0:00:00 |
Molecular identification of Tomato chlorosis virus infecting tomato in Guangdong Province |
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| Tang Yafei,He Zifu,She Xiaoman,Lan Guobing.Molecular identification of Tomato chlorosis virus infecting tomato in Guangdong Province[J].Plant Protection,2017,43(2):133-137. |
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| Authors: | Tang Yafei He Zifu She Xiaoman Lan Guobing |
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| Institution: | 1. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 2. Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China |
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| Abstract: | A new disease on tomato recently occurs in Guangdong Province. The symptoms of the diseased tomato plants include leaf chlorotic, vein dark-greening and leaf thickening. The expected DNA fragments were amplified from all 6 diseased samples with two specific primer pairs for HSP70 gene of Tomato chlorosis virus (ToCV) by RT-PCR. And a full length of HSP70 (1 665 bp) was obtained by sequencing, which shared high nucleotides identity with those from other ToCV isolates. The result indicated that the collected tomato samples have been infected by ToCV in Guangdong. But there exists great difference in nucleotide sequence of HSP70 between the isolate from Guangdong (GD01) and other ToCV isolates from all over the world. The nucleotide sequence of HSP70 from isolate GD01 shared the highest identity (81.8%) with isolate tomato from Cyprus and JU_20 from Jordan. This paper was the first report on the occurrence of ToCV in Guangdong, and also the first report of significant difference in nucleotide sequence of HSP70 between ToCV GD01 and other isolates. |
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| Keywords: | tomato viral disease Tomato chlorosis virus HSP70 gene |
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