| 强还原处理所使用有机物料与其杀菌效果的相互关系 |
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| 引用本文： | 刘亮亮,崔慧灵,孔继婕,张金波,蔡祖聪,黄新琦.强还原处理所使用有机物料与其杀菌效果的相互关系[J].植物保护,2017,43(2):73-81. |
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| 作者姓名： | 刘亮亮 崔慧灵 孔继婕 张金波 蔡祖聪 黄新琦 |
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| 作者单位： | 1. 南京师范大学地理科学学院,南京,210023;2. 南京师范大学地理科学学院,南京210023;虚拟地理环境教育部重点实验室(南京师范大学),南京210023;江苏省地理环境演化国家重点实验室培育建设点,南京210023;江苏省物质循环与污染控制重点实验室,南京210023;江苏省地理信息资源开发与利用协同创新中心,南京210023 |
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| 基金项目： | 国家自然科学基金(41301335) |
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| 摘 要： | 创造土壤厌氧环境并添加大量有机物料的强还原处理(reductive soil disinfestation,RSD)是一种有效的土壤消毒方法,但对于RSD过程中添加不同有机物料与其杀菌效果间的相互关系研究甚少。本试验采集两种连作土壤,分别进行添加稻壳(RSD1)、麦麸(RSD2)和芦苇(RSD3);甘蔗渣(RSD4)、葡萄糖(RSD5)和纤维素(RSD6)的RSD处理,采用荧光定量PCR、变性梯度凝胶电泳等方法研究物料理化性质与杀菌效果的相互关系。结果表明:RSD处理显著地降低了土壤中的NO-3-N含量和电导率Ec,增加了NH+4-N含量;稻壳、麦麸和芦苇RSD处理的杀菌效果分别为88.60%、90.76%和85.91%,甘蔗渣、葡萄糖和纤维素RSD处理的杀菌效果分别为72.30%、85.87%和30.65%;有机物料中的总有机碳(total organic carbon,TOC)、易氧化有机碳(easily oxidized organic carbon,EOC)和总氮(total nitrogen,TN)与杀菌效果及细菌数量都呈显著正相关(P0.05),土壤中添加有机物料初始C/N与杀菌效果呈显著负相关,与细菌数量显著正相关。综上所述,添加高EOC和TN及低C/N的有机物料的RSD处理杀菌效果更好。
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| 关 键 词： | 强还原处理 杀菌效果 有机物料 细菌多样性 |
| 收稿时间： | 2016/4/14 0:00:00 |
| 修稿时间： | 2016/5/25 0:00:00 |
Relationships between different types of organic matters and disinfestation effects during reductive soil disinfestation |
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| Liu Liangliang,Cui Huiling,Kong Jijie,Zhang Jinbo,Cai Zucong,Huang Xinqi.Relationships between different types of organic matters and disinfestation effects during reductive soil disinfestation[J].Plant Protection,2017,43(2):73-81. |
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| Authors: | Liu Liangliang Cui Huiling Kong Jijie Zhang Jinbo Cai Zucong Huang Xinqi |
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| Institution: | 1. College of Geography, Nanjing Normal University, Nanjing 210023, China; 2. Key Laboratory of Virtual Geographic Environment (Nanjing Normal University), Ministry of Education, Nanjing 210023, China; 3. State Key Laboratory Cultivation Base of Geographical Environmental Evolution (Jiangsu Province), Nanjing 210023, China; 4. Jiangsu Provincial Key Laboratory of Material Cycling and Pollution Control, Nanjing 210023, China; 5. Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing 210023, China |
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| Abstract: | Although reductive soil disinfestation (RSD) through creating anaerobic condition and incorporating soil with large amounts of organic matters is an effective method for soil disinfestation, there is limited information about the relationships between the type of organic matter and disinfestation effects (DEs). Hence, in this study, various RSD treatments incorporated with rice hull (RSD1), wheat bran (RSD2), and reed (RSD3), sugarcane residue (RSD4), glucose (RSD5), and cellulose (RSD6) were respectively conducted, and quantitative real-time PCR and denaturing gradient gel electrophoresis (DGGE) were performed to investigate the relationships between organic matters and DEs. The results showed that the content of soil NO-3-N and electrical conductivity (Ec) significantly decreased in the RSD treatments, whereas the content of NH+4-N significantly increased. The DEs of these RSD treatments (RSD1-6) were 88.60%, 90.76%, 85.91%, 72.3%, 85.87%, and 30.65%, respectively. Total organic carbon (TOC), easily oxidized organic carbon (EOC), and total nitrogen (TN) in the organic matter had a significant positive correlation with DE and bacterial population (P<0.05). Besides, the initial soil C/N after incorporating the organic matters had a significant negative correlation with DE, and had a significant positive correlation with bacterial population. Therefore, the RSD treatments incorporated with the organic matters with higher EOC, TN and lower C/N would obtain a better disinfestation effect. |
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| Keywords: | reductive soil disinfestation disinfestation effect organic matter bacterial diversity |
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