| 交枝顶孢霉杀柑橘全爪螨活性及其生物学特性研究 |
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| 引用本文： | 王 娟,胡军华,龙艳玲,周 娜,彭凤格,姚廷山,李鸿筠.交枝顶孢霉杀柑橘全爪螨活性及其生物学特性研究[J].植物保护,2017,43(2):95-101. |
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| 作者姓名： | 王 娟 胡军华 龙艳玲 周 娜 彭凤格 姚廷山 李鸿筠 |
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| 作者单位： | 西南大学柑桔研究所/国家柑桔工程技术研究中心/西南果树科学观测实验站,重庆,400712 |
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| 基金项目： | 公益性行业(农业)科研专项(201103020); 重庆市科技支撑示范工程(cstc2014fazktjcsf0075); “十二五”农村领域国家科技计划课题(2014BAD16B702-5) |
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| 摘 要： | 为了明确交枝顶孢霉Acremonium implicatum CQBBW8的杀螨活性及其生物学特性,采用触杀毒力法检测CQBBW8菌株对柑橘全爪螨的致死率,通过单因素试验测试不同培养基、碳源、氮源、温度、pH及紫外线对CQBBW8菌株生长、产孢及其分生孢子萌发的影响。结果表明,CQBBW8菌株对柑橘全爪螨Panonychus citri(McGregor)的校正致死率为54.42%,LC_(50)为1.2×10~6个/mL,LT_(50)值为5.175 9d。最佳生长和产孢培养基分别为PDA和SEA;菌丝生长最适碳源和氮源为甘露醇和蛋白胨,产孢最适碳源和氮源为可溶性淀粉和氯化铵;最适生长温度为25℃,最适产孢和分生孢子萌发温度为30℃;最适生长和产孢pH为6.0,最适萌发pH为7.0;紫外线对菌丝生长影响不明显,对产孢影响较大,紫外线照射时间越长,孢子萌发率越高。综上,CQBBW8菌株对柑橘全爪螨有较强的毒力,对营养需求不高,环境适应性较强,具有开发为杀螨真菌制剂的潜力。
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| 关 键 词： | 交枝顶孢霉 生物学特性 杀螨活性 |
| 收稿时间： | 2016/4/20 0:00:00 |
| 修稿时间： | 2016/6/10 0:00:00 |
Biological characteristics and acaricidal activity of Acremonium implicatum to Panonychus citri (McGregor) |
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| Wang Juan,Hu Junhu,Long Yanling,Zhou N,Peng Fengge,Yao Tingshan,Li Hongjun.Biological characteristics and acaricidal activity of Acremonium implicatum to Panonychus citri (McGregor)[J].Plant Protection,2017,43(2):95-101. |
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| Authors: | Wang Juan Hu Junhu Long Yanling Zhou N Peng Fengge Yao Tingshan Li Hongjun |
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| Institution: | Citrus Research Institute, Southwest University, National Engineering Research Center for Citrus, Scientific Observation and Experimental Station of Fruit Tree Science (Southwest Region), Ministry of Agriculture, Chongqing 400712, China |
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| Abstract: | To explore the acaricidal activity and biological characteristics of Acremonium implicatum CQBBW8,the lethal rate of CQBBW8 strain to Panonychus citri (McGregor) was detected by using contact toxicity method,and the effects of different factors,such as culture medium,carbon source,nitrogen source,temperature,pH,and UV on the hyphal growth,sporulation and spore germination of A.implicatum were tested by using single factor test.The results indicated that the corrected mortality rate of CQBBW8 strain to P.citri was 54.42％;the LC50 was 1.2× 106 spores/mL and the LT50 was 5.175 9 d.The optimum growth and sporulation culture medium were PDA and SEA.Mannite and peptone were the best carbon and nitrogen sources for hyphal growth,and soluble starch and ammonium chloride were the best carbon and nitrogen sources for sporulation,respectively.The most suitable culture temperature for hyphal growth was 25℃,and the most suitable temperature for sporulation and spore germination was 30℃.The optimum pH for hyphal growth and sporulation was 6.0,and the optimum pH for spore germination was 7.0.Although UV had no significant effect on fungal growth rate,it had a marked effect on sporulation;the longer the ultraviolet irradiation time,the higher the rate of spore germination.In summary,CQBBW8 strain had a strong toxicity to P.citri,without a high nutritional demand,and its environmental adaptability was strong,with a potential for development of the fungus preparation for killing mites. |
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| Keywords: | Acremonium implicatum biological characteristic acaricidal activity |
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