| 胶体金免疫层析法测定动物组织中氯霉素残留 |
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| 引用本文： | 陶光灿,吴鹏,赵正苗,崔廷婷,冯静.胶体金免疫层析法测定动物组织中氯霉素残留[J].吉林畜牧兽医,2012,33(6):21-23. |
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| 作者姓名： | 陶光灿 吴鹏 赵正苗 崔廷婷 冯静 |
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| 作者单位： | 北京勤邦生物技术有限公司,北京,102206 |
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| 摘 要： | 应用胶体金免疫层析法对动物组织中的氯霉素残留进行检测,研究该方法的灵敏度、假阳性率、假阴性率及特异性。结果表明,用胶体金免疫层析法测定猪肉、鸡肉、鱼、虾中的氯霉素,检测限均为0.3g/kg,假阳性率和假阴性率皆为0%;方法的特异性较好,对其他抗生素无交叉反应;检测加标样本的结果与液相色谱-串联质谱法基本一致。可见,该方法简便、快速、准确、稳定,可作为动物组织中氯霉素残留现场快速检测的一种有效手段。
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| 关 键 词： | 动物组织 氯霉素残留 胶体金免疫层析法 |
Detection of Chloramphenicol Residues in Animal Tissues by Gold Immunochromatographic Assay |
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| TAO Guang-can,WU Peng,ZHAO Zheng-miao,CUI Ting-ting,FENG Jing.Detection of Chloramphenicol Residues in Animal Tissues by Gold Immunochromatographic Assay[J].Jilin Animal Science and Veterinary Medicine,2012,33(6):21-23. |
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| Authors: | TAO Guang-can WU Peng ZHAO Zheng-miao CUI Ting-ting FENG Jing |
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| Institution: | Beijing Kwinbon Biotechnology Co., Ltd., Beijing 102206 |
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| Abstract: | A gold immunochromatographic assay （GICA） was used to detect the residues of chloramphenicol in animal tis- sues. The sensitivity, false positive rate （FPR）, false negative rate （FNR） and specificity of the method were studied. The re- suits showed that the detect limit of GICA was 0.3pg/kg. FPR and FNR were both 0%. The specificity was good and there was no cross-reactivity with any other antibiotics. The results of GICA and liquid chromatographic tandem mass spectro- metry （LC-MS/MS） were in accordance. The method is simple, rapid, accurate and stable, and suitable for the on-site rapid detection of chloramphenicol residues in animal tissues. |
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| Keywords: | animal tissues chloramphenicol residues gold immunochromatographic assay (GICA) |
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