| 黄瓜复雌花性状QTL定位分析 |
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| 引用本文： | 苗晗,顾兴芳,张圣平,武剑,方智远,张振贤.黄瓜复雌花性状QTL定位分析[J].园艺学报,2010,37(9):1449-1455. |
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| 作者姓名： | 苗晗 顾兴芳 张圣平 武剑 方智远 张振贤 |
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| 作者单位： | （1中国农业大学农学与生物技术学院，北京 100193；2中国农业科学院蔬菜花卉研究所，北京 100081） |
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| 基金项目： | 国家现代农业产业技术体系建设专项，国家'948'项目，国家'973'计划项目，国家'863'计划项目，农业部园艺作物遗传改良重点开放实验室项目 |
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| 摘 要： | 利用具有复雌花性状的华北保护地类型黄瓜材料9930和具有单雌花性状的欧洲温室类型黄瓜材料9110Gt构建的含148个株系的F9代RILs群体进行QTL定位分析。利用已构建的遗传图谱，使用MapQTL4.0软件进行多座位QTL模型（MQM）检测。共检测到8个控制复雌花性状的QTL，分布在第1、2、3、6、7染色体上。各QTL的LOD值在3.62 ~ 8.37之间，可解释8.2% ~ 20.0%的表型变异。6个QTL贡献率超过10%，位于第3染色体的Mp3.2贡献率（2007秋季20.0%，LOD = 8.37）最大；位于第6染色体的Mp6.2（2006春季13.9%，LOD = 5.95；2007秋季11.9%，LOD = 5.10；2009春季11.5%，LOD = 4.38）在两季的位置都很稳定。获得紧密连锁（< 10 cM）的特异标记（SSR04635、SSR13466、SSR00584、SSR10449）为将来进行QTL精细定位提供了参考。
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| 关 键 词： | 黄瓜 复雌花 QTL 重组自交系 |
| 收稿时间： | 2010-03-22 |
Mapping QTLs for Multiple Pistillate Flowers in Cucumber |
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| MIAO Han,GU Xing-fang,ZHANG Sheng-ping,WU Jian,FANG Zhi-yuan,ZHANG Zhen-xian.Mapping QTLs for Multiple Pistillate Flowers in Cucumber[J].Acta Horticulturae Sinica,2010,37(9):1449-1455. |
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| Authors: | MIAO Han GU Xing-fang ZHANG Sheng-ping WU Jian FANG Zhi-yuan ZHANG Zhen-xian |
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| Institution: | （1College of Agriculture and Biotechnology，China Agricultural University，Beijing 100193，China；2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China） |
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| Abstract: | Multiple pistillate flowers trait is related closely to cucumber（Cucumis sativus L.）production. This study was made to map and analyze QTLs of multiple pistillate flowers traits in cucumber，so as to provide information for molecular marker assisted selection of cucumber breeding for high yield. A linkage map of cucumber was constructed using 148 F9 recombinant inbred lines（RILs）originated from a narrow-cross between 9110Gt（single pistillate flower，SP）and 9930（multiple pistillate flowers，MP）in the experiment. Total of 8 QTLs were detected using the genetic map and multiple QTL model（MQM）method of software package MapQTL version 4.0. These QTLs were mapped on chromosome 1，2，3，6，7，respectively. Their LOD values varied between 3.62 and 8.37 which explaining 8.2%–20.0% of the phenotypic variation. Six QTLs explained phenotypic variation more than 10% which Mp3.2 explained highest phenotypic variation（2007 autumn 20.0%，LOD = 8.37），and Mp6.2（2006 spring 13.9%，LOD = 5.95；2007 autumn 11.9%，LOD = 5.10；2009 spring 11.5%，LOD = 4.38）was detected in the two seasons. The tight linkage markers（SSR04635，SSR13466，SSR00584 and SSR10449）can be used in fine mapping of multiple pistillate flowers in cucumber. |
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| Keywords: | QTL |
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