| 鸭病毒性肠炎病毒强毒在人工感染鸭体内形态结构和发生学的电镜观察 |
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| 引用本文： | 袁桂萍,程安春,汪铭书,周毅,刘菲,韩晓英,郭宇飞,廖永洪,徐超,文明,贾仁勇,周伟光,陈孝跃.鸭病毒性肠炎病毒强毒在人工感染鸭体内形态结构和发生学的电镜观察[J].畜牧兽医学报,2005,36(5):486-491. |
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| 作者姓名： | 袁桂萍 程安春 汪铭书 周毅 刘菲 韩晓英 郭宇飞 廖永洪 徐超 文明 贾仁勇 周伟光 陈孝跃 |
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| 作者单位： | 四川农业大学动物科技学院禽病防治研究中心,动物疫病与人类健康四川省重点实验室,雅安,625014 |
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| 基金项目： | 四川杰出青年学科带头人基金后续资助项目(03ZQ026 028)，四川省重点建设学科项目(SZD0418) |
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| 摘 要： | 为研究鸭病毒性肠炎病毒(DEV)CH强毒株在感染鸭体内的分布和形态学发生规律，应用透射电镜和超薄切片技术对人工感染DEV的成年鸭各组织器官进行观察。结果表明：感染后12h在脾脏和法氏囊首先观察到少量的DEV出现，24h后在脾、胸腺和法氏囊以及死亡鸭的肝、肠和胰中均观察到具有典型的疱疹病毒粒子及其核衣壳形态的DEV。DEV病毒核衣壳有空心型、致密核心型、双环型和内壁附有颗粒型4种形态，存在胞核和胞浆两种装配方式。病毒成熟有两种方式：一为细胞核内核衣壳在核内获得皮层，通过核内膜获得囊膜成为成熟病毒；二为核内核衣壳通过内外核膜进入胞浆，核内和胞浆内的核衣壳在细胞浆中获得皮层，然后在各种质膜上获得囊膜，最后成熟病毒通过细胞破裂或其他方式释放到细胞外。伴随着病毒的复制、装配和成熟，细胞中出现多种核内和胞浆包涵体、核内致密颗粒、核内微管和中空短管、胞浆电子致密小体等结构。
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| 关 键 词： | 病毒性肠炎 人工感染 电镜观察 形态结构 体内 发生学 超薄切片技术 核衣壳 发生规律 组织器官 透射电镜 病毒粒子 装配方式 致密颗粒 法氏囊 胞浆 成熟 形态学 强毒株 成年鸭 致密核 颗粒型 细胞核 细胞外 包涵体 皮层 |
| 文章编号： | 0366-6964(2005)05-0486-06 |
Electron Microscopic Studies on Morphology and Morphogenesis of Duck Enteritis Virus in Ducks Infected with DEV Virulent Strain Experimentally |
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| Yuan Gui-ping,CHENG An-chun,WANG Ming-shu,ZHOU Yi,LIU Fei,HAN Xiao-ying,GUO Yu-fei,LIAO Yong-hong,XU Chao,WEN Ming,JIA Ren-yong,ZHOU Wei-guang,CHEN Xiao-yue.Electron Microscopic Studies on Morphology and Morphogenesis of Duck Enteritis Virus in Ducks Infected with DEV Virulent Strain Experimentally[J].Acta Veterinaria et Zootechnica Sinica,2005,36(5):486-491. |
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| Authors: | Yuan Gui-ping CHENG An-chun WANG Ming-shu ZHOU Yi LIU Fei HAN Xiao-ying GUO Yu-fei LIAO Yong-hong XU Chao WEN Ming JIA Ren-yong ZHOU Wei-guang CHEN Xiao-yue |
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| Abstract: | In order to study the morphogenesis and distribution of duck enteritis virus(DEV),tissues of ducks infected experimentally with DEV virulent strain were collected and observed by electron microscopy.The investigation showed that a few typical herpesvirus virions and nucleocapsids were observed in the spleen and bursa of Fabricius 12 hours post inoculation,then,in the liver,small intestine,spleen, thymus,bursa of Fabricius 24 hours post inoculation.Nucleocapsids are divided into four types according to their morphology. Nucleocapsids assembled both in nucleus and cytoplasm.There are two types of maturation according to our observation.One is nucleocapsids obtained its tegument in the nucleus and enveloped from the inner nuclear membrane.Another is nucleocapsids entered cytoplasm through nuclear membrane,then obtained their tegument in the cytoplasm,enveloped from the plasma membrane,finally released by necrosis,exocytosis or other ways.With the replication,assembly and maturation of viruses,inclusion bodies,compact particles, microtubes,hollow tubes and coated electron dense bodies are observed. |
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| Keywords: | duck enteritis virus artificial infection morphogenesis |
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