| 鸡源性多重耐药沙门氏菌 类整合子与耐药基因研究 |
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| 引用本文： | 赵玉林,刘芳萍,李昌文,刘立新,李睿,罗鹏志,卢斯亮,张秀英.鸡源性多重耐药沙门氏菌 类整合子与耐药基因研究[J].中国家禽,2012,34(4):29-32. |
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| 作者姓名： | 赵玉林 刘芳萍 李昌文 刘立新 李睿 罗鹏志 卢斯亮 张秀英 |
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| 作者单位： | 1. 东北农业大学动物医学学院,黑龙江哈尔滨,150030
2. 中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001 |
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| 基金项目： | 黑龙江省教育厅自然科学面上项目 |
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| 摘 要： | 本试验旨在了解鸡源性沙门氏菌分离株多重耐药与Ⅰ类整合子及耐药基因的携带关系。采用K-B纸片法对29株鸡源性沙门氏菌分离菌株进行10种抗菌药物敏感试验;应用PCR技术对分离菌株进行Ⅰ类整合子及耐药基因检测。29株分离株中有13株对2种以上抗菌药物耐药,属于多重耐药株,氨苄西林-四环素-头孢唑啉-复合磺胺是主要多重耐药谱;13株多重耐药菌中有8株携带Ⅰ类整合子,blatem-1、tetA和tetB基因检出最高。结果表明沙门氏菌多重耐药性与整合子的携带之间关系密切,耐药表型测定结果与耐药基因检测结果基本一致。
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| 关 键 词： | 鸡 多重耐药 沙门氏菌 Ⅰ类整合子 耐药基因 |
Detection of Integron Ⅰ and Drug Resistance Genes of Multidrug-resistance Salmonella Isolated from Chickens |
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| ZHAO Yulin
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LIU Fangping
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LI Changwen
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LIU Lixin
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LI Rui
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LUO Pengzhi
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LU Siliang
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ZHANG Xiuying.Detection of Integron Ⅰ and Drug Resistance Genes of Multidrug-resistance Salmonella Isolated from Chickens[J].China Poultry,2012,34(4):29-32. |
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| Authors: | ZHAO Yulin LIU Fangping LI Changwen LIU Lixin LI Rui LUO Pengzhi LU Siliang ZHANG Xiuying |
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| Institution: | 1 (1.College of Veterinary Medicine,Northeast Agricultural University,Harbin,Heilongjiang 150030; 2. Harbin Veterinary Research Institute of CAAS,Harbin,Heilongjiang 150001) |
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| Abstract: | To determine the relationship between integron Ⅰ and drug resistance genes multidrug-resistance Salmonella isolated from chickens,the sensitivity tests of 29 strains to 10 antibacterials were performed by Kirby-Bauer method,the integron Ⅰ and resistance genes were detected by PCR. There were 13 strains were multidrug resistance strains which showed resistance to more than two kinds of antibacterials. Ampicillin-Tetracycline-Cefazolin Compound Sulfadiazine was the main multidrug resistance spectrum,and there were 8 multidrug resistance strains carried integron Ⅰ,and blatme-1,tetA and tetB were the predominant genes. These results suggested that multidrug-resistance Salmonella had a close correlation with carried integron,the detection of resistance phenotype agreed with the detection of resistance genes. |
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| Keywords: | chicken multidrug resistance Salmonella integron Ⅰ resistance genes |
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