Potential Precursor of Angiotensin-I Converting Enzyme (ACE) Inhibitory Activity and Structural Properties of Peptide from Peptic Hydrolysate of Cutlassfish Muscle |
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| Authors: | Hyun-Soo Kim WonWoo Lee Thilina U Jayawardena Nalae Kang Min Cheol Kang Seok-Chun Ko |
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| Institution: | 1. Department of Genetic Resources Research, National Marine Biodiversity Institute of Korea , Janghang-eup, Republic of Korea;2. Freshwater Bioresources Utilization Division, Nakdonggang National Istitute of Biological Resources , Sangju, Republic of Korea;3. Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University , Jeju, Republic of Korea;4. Jeju Marine Research Center, Korea Institute of Ocean Science &5. Technology (KIOST) , Jeju, Korea;6. Research Group of Food Processing, Korea Food Research Institute , Wanju-gun, Korea;7. National Marine Bio-resources and Information Center, National Marine Biodiversity Institute of Korea , Seochun, Republic of Korea |
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| Abstract: | ABSTRACT Peptic hydrolysates were prepared by digesting the cutlassfish muscle protein using pepsin for 1, 3, and 6 h, and their inhibitory activity against angiotensin-I converting enzyme (ACE) was studied. The ACE-inhibitory effect of the peptic hydrolysate of cutlassfish muscle generated at the 3 h time point exhibited the strongest activity. After identifying the optimal hydrolysate, the active peptide was isolated by ultrafiltration, gel permeation, and high performance liquid chromatography (HPLC). The resulting purified peptide was characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS/MS) and was identified to be a 496.44 Da pentapeptide (Phe-Ser-Gly-Gly-Glu). The ACE-inhibitory activity of the active peptide exhibited an IC50 value of 0.033 ± 0.003 mg/ml. A molecular docking program was used to simulate the interaction between the peptide and ACE, which revealed that the inhibitory effect was mainly due to the hydrogen bonds between ACE and the peptide. Based on the ACE-inhibitory properties and the molecular docking study of the resulting active peptide, we demonstrated an increase in nitric oxide (NO) production in a dose-dependent manner. In conclusion, cutlassfish protein hydrolysate and the resulting active peptide could be used as active ingredients in functional food as anti-hypertensive agents. |
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| Keywords: | Cutlassfish Angiotensin-I converting enzyme (ACE) inhibitory peptide peptic hydrolysate hypertension |
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