Antimicrobial susceptibility and molecular characterization of macrolide resistance of
Mycoplasma bovis isolates from multiple provinces in China |
| |
| Authors: | Ling-Cong KONG Duo GAO Bo-Yan JIA Zi WANG Yun-Hang GAO Zhi-Hua PEI Shu-Ming LIU Jiu-Qing XIN Hong-Xia MA |
| |
| Institution: | 1)College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No.#2888, Changchun 130118, P.R. China;2)National Contagious Bovine Pleuropneumonia Reference Laboratory, Division of Bacterial Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin 150000, P.R. China |
| |
| Abstract: | Mycoplasma bovis has spread widely throughout the world via animal movement and has become
an important pathogen of bovine respiratory disease. However, the minimum inhibitory concentrations of
antimicrobials for Mycoplasma bovis have not been studied in China. The objective of this
study was to determine the prevalence and antibiotic resistance of Mycoplasma bovis isolated
from young cattle with respiratory infection in China. Mycoplasma bovis was detected in 32/45
bovine respiratory infection outbreaks at beef farms in 8 provinces in China. The isolates were susceptible or
had medium sensitivity to ciprofloxacin, enrofloxacin and doxycycline, but were frequently resistant to
macrolides (13/32, 41%). An A2058G (Escherichia coli Numbering) mutation located in the
rrnA operon in domain V of 23S rRNA was observed in strains that were resistant to
macrolides. This single mutations at the rrnA operon in domain V of 23S rRNA may play an important role in the
resistance of Mycoplasma bovis strains to macrolides. |
| |
| Keywords: | antimicrobial susceptibility bovine respiratory infection macrolides mycoplasma bovis target mutation |
|
|
