|
|||||
|
|
| 非离子表面活性剂PEG对棉秆木质纤维素酶解的影响 | |
| 引用本文： | 席琳乔,吴书奇,马春晖.非离子表面活性剂PEG对棉秆木质纤维素酶解的影响[J].农业工程学报,2012,28(23):176-183. |
| 作者姓名： | 席琳乔 吴书奇 马春晖 |
| 作者单位： | 1. 东北农业大学动物科学学院,哈尔滨 150030 2. 塔里木大学动物科学学院,阿拉尔 843300 3. 新疆生产建设兵团塔里木畜牧科技重点实验室,阿拉尔 843300 |
| 基金项目： | 国家自然基金（3096026） |
| 摘 要： | 为了提高纤维素的水解作用,减少酶的用量,该文研究不同分子量的聚乙二醇(PEG,poly ethylene glycol)表面活性剂对棉秆木质纤维素水解复合酶的影响,研究不同分子量的PEG对还原糖、纤维素酶、木质素酶、蛋白吸附率、酶动力学特征的影响。结果表明,2.5g/L添加量的PEG 2000、PEG 4000、PEG 6000和PEG 8000均能提高棉秆木质纤维素水解酶的活力,增加纤维素的转化率,减少蛋白的非特异性吸附率,提高棉秆木质素和纤维素的降解率,提高最大反应速度和降低酶与底物的亲和力,其中PEG 6000效果最好。3.0g/L的PEG 6000添加量对纤维素的转化率最高,达到58.12%(P<0.05)。对于吸附率,2.0、3.0、4.0、5.0g/L的添加量与对照相比差异显著(P<0.05),且相互之间差异不显著(P>0.05)。添加3.0g/L PEG 6000的表面活性剂能保持上清液中较高的蛋白浓度,水解3h后,没有添加表面活性剂的处理,纤维滤纸酶酶活从0.1245FPU/mL下降到0.012FPU/mL,下降了90.37%,添加PEG 6000后,FPA酶的活性恢复到0.041FPU/mL;水解48h,添加PEG 6000和不添加PEG 6000纤维素的转化率分别达到65.71%和54.68%,添加PEG 6000提高纤维素转化率20.18%。结果表明,PEG6000可以提高纤维素的转化率,为工业生产实践提供了参考。 |
| 关 键 词： | 秸秆 水解 酶 表面活性剂 PEG |
| 收稿时间： | 2012/3/27 0:00:00 |
| 修稿时间： | 2012/10/7 0:00:00 |
Effect of nonionic surfactant PEG on lignocellulose enzymatic hydrolysis of cotton stalk |
|
| Xi Linqiao,Wu Shuqi and Ma Chunhui.Effect of nonionic surfactant PEG on lignocellulose enzymatic hydrolysis of cotton stalk[J].Transactions of the Chinese Society of Agricultural Engineering,2012,28(23):176-183. | |
| Authors: | Xi Linqiao Wu Shuqi and Ma Chunhui |
| Institution: | 2,3※(1.College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China;2.College of Animal Science and Technology,Tarim University,Arael 843300,China;3.Xinjiang Production and Construction Crops Key Laboratory of Tarim Animal Husbandry Science and Technology,Arael 843300,China) |
| Abstract: | In order to improve the cellulose hydrolysis, reducing the amount of enzyme, the effect of different degree ploymerization poly ethylene glycol (PEG, with various amount of ethylene oxide) on the enzymatic hydrolysis of cotton stalk was investigated, especially with regard to soluble reducing sugar, enzyme activity, enzyme protein adsorption, degradation rate, and enzyme kinetics parameters. The results showed that addition 2.5 g/L of PEG2000, 4000, 6000, 8000 binded to lignin by hydrophobic interaction and hydrogen bonding, reduced the unproductive binding of enzymes, in which PEG6000 was the best. Addition of 3.0 g/L of PEG6000 to enzyme hydrolysis of lignocellulose increased the conversion rate from 28.13% to 58.12% with 24 h hydrolysis time. Adsorption of enzyme protein content decreased from78.72% to 45.17%. By addition of PEG6000 it was possible to perform hydrolysis in 3 h. FPA (filter paper activity) decreased from 0.1245 to 0.012 FPU/mL, and descended by 90.37% without PEG6000. FPA was recovered from 0.012 to 0.041 FPU/mL with PEG6000. With 48 h hydrolysis, conversion rate with and without PEG6000 addition reached 65.71% and 54.68%, respectively, which was improved by 18%. PEG6000 addition can improve the cotton stalk lignocellulose hydrolysis rate, and the results can provide a reference for the industrial production of cotton stalk. |
| Keywords: | straw hydrolysis enzymes surfactants PEG |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《农业工程学报》浏览原始摘要信息 | |
| 点击此处可从《农业工程学报》下载免费的PDF全文 | |