| 酶制剂对水稻秸秆青贮发酵品质及体外消化特性的影响 |
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| 引用本文： | 吴永杰,丁浩,邵涛,赵杰,董东,代童童,尹雪敬,宗成,李君风.酶制剂对水稻秸秆青贮发酵品质及体外消化特性的影响[J].草业学报,2022,31(8):167-177. |
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| 作者姓名： | 吴永杰 丁浩 邵涛 赵杰 董东 代童童 尹雪敬 宗成 李君风 |
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| 作者单位： | 南京农业大学草业学院饲草调制加工与贮藏研究所，江苏 南京 210095 |
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| 基金项目： | 国家自然科学基金区域创新发展联合基金重点支持项目(U20A2003) |
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| 摘 要： | 为探讨纤维素酶、木聚糖酶及两种酶组合添加对水稻秸秆青贮过程中结构性、水溶性碳水化合物组分含量及体外消化特性和发酵品质的影响，试验设4个处理组：1）0.3%蒸馏水（对照组，CO）；2）0.3%纤维素酶（CE）；3）0.3%木聚糖酶（XE）；4）0.15%纤维素酶+0.15%木聚糖酶（组合酶组，CX），分别于青贮3、7、14、30 d后取样分析。结果表明，与CO相比，CE、XE和CX组显著提高了乳酸、葡萄糖、果糖和蔗糖含量，显著降低了pH值、氨态氮、总挥发性脂肪酸、酸性洗涤纤维、中性洗涤纤维、纤维素和半纤维素含量，减少了干物质损失（P<0.05）。酶制剂显著提高了水稻秸秆青贮饲料24、48和72 h时的累积产气量和干物质体外消化率（P<0.05）。青贮末期CX组有最高的乳酸含量（34.13 g·kg-1 DM）、体外产气量（68.27 mL）、干物质体外消化率（61.31%）和最低的pH值（4.36）。与CE和XE相比，CX组水溶性碳水化合物（葡萄糖、果糖和蔗糖）含量更高。综上所述，添加酶制剂可促进结构性碳水化合物的降解，提高水溶性碳水化合物的含量，改善水稻秸秆青贮发酵品质及体外消化率，与CE和XE相比，CX组发酵品质、碳水化合物的降解转化和体外消化特性更好。
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| 关 键 词： | 纤维素酶 木聚糖酶 水稻秸秆 发酵品质 体外消化特性 |
| 收稿时间： | 2021-08-19 |
| 修稿时间： | 2021-11-22 |
Effects of enzyme additives on fermentation quality and in vitro digestion characteristics of rice straw silage |
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| Yong-jie WU,Hao DING,Tao SHAO,Jie ZHAO,Dong DONG,Tong-tong DAI,Xue-jing YIN,Cheng ZONG,Jun-feng LI.Effects of enzyme additives on fermentation quality and in vitro digestion characteristics of rice straw silage[J].Acta Prataculturae Sinica,2022,31(8):167-177. |
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| Authors: | Yong-jie WU Hao DING Tao SHAO Jie ZHAO Dong DONG Tong-tong DAI Xue-jing YIN Cheng ZONG Jun-feng LI |
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| Institution: | Institute of Ensiling and Processing of Grass，College of Agro-grassland Science，Nanjing Agricultural University，Nanjing 210095，China |
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| Abstract: | This research investigated the effects of cellulase， xylanase and their combination on the structural and water-soluble carbohydrate components， in vitro digestion characteristics and fermentation quality of rice straw silage. Four treatments were set up as follows： 1） 0.3% distilled water， control （CO）； 2） 0.3% cellulase （CE）； 3） 0.3% xylanase （XE）； 4） a mixture of 0.15% cellulase and 0.15% xylanase， combined enzyme group （CX）. Samples were taken and analyzed after 3， 7， 14 and 30 days of ensiling. Compared to the CO treatment， CE， XE and CX treatments had significantly （P<0.05） higher lactic acid， glucose， fructose and sucrose contents， but significantly （P<0.05） lower pH values， ammonia nitrogen， total volatile fatty acid， acid detergent fiber， neutral detergent fiber， cellulose and hemicellulose contents and dry matter loss. The enzyme additives significantly （P<0.05） increased the cumulative gas production at 24， 48 and 72 hours and in vitro dry matter digestibility of rice straw silage. The CX treatment had the highest （P<0.05） lactic acid content （34.13 g·kg-1 DM）， in vitro gas production （68.27 mL）， in vitro dry matter digestibility （61.31%） and the lowest pH value （4.36） at the end of ensiling. In addition， the CX group had numerically （P>0.05） or significantly （P<0.05） higher glucose， fructose and sucrose contents than CE and XE groups during ensiling. In summary， the addition of enzymes promoted the degradation of structural carbohydrates， increased the water-soluble carbohydrate content， and improved the fermentation quality and in vitro digestibility of rice straw silage. The CX treatment performed better than the CE and XE treatments， as indicated by superior fermentation quality， higher carbohydrate degradation and conversion， and better in vitro digestion characteristics. |
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| Keywords: | cellulase xylanase rice straw fermentation quality in vitro digestion characteristics |
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