| 葛根素对小白菜镉损伤的保护作用研究 |
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| 引用本文： | 胡远锋,张明,范淑英,葛菲,朱卫丰,肖旭峰.葛根素对小白菜镉损伤的保护作用研究[J].核农学报,2023,37(2):424-431. |
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| 作者姓名： | 胡远锋 张明 范淑英 葛菲 朱卫丰 肖旭峰 |
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| 作者单位： | 1.江西农业大学农学院，江西 南昌 3300452.江西生物科技职业学院动物科学系，江西 南昌 3302003.江西中医药大学药学院，江西 南昌 330004 |
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| 基金项目： | 国家自然科学基金项目(31860560);江西省现代农业产业技术体系建设专项(JXARS-16);国家重点研发计划项目(2017YFC1702901);江西中医药大学中药学一流学科科研项目(JXSYLXK-ZHYAO024) |
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| 摘 要： | 为探究葛根素(PUE)对小白菜镉(Cd)损伤的保护效果，采取外源添加不同葛根素处理，将小白菜植株随机分为7组，即对照组(CK)、PUE30组(葛根素30μmol·L-1)、Cd4组(镉4 mg·L-1)、Cd与PUE共处理组(4+15、4+30、4+45、4+60)。处理30 d后，观察小白菜植株生长状况、测定Cd富集量及抗胁迫生理活性物质，包括叶绿素、抗氧化酶(SOD、APX、CAT、POD)活性及过氧化氢(H2O2)、丙二醛(MDA)Vc、可溶性蛋白含量，并用实时荧光定量PCR(qRT-PCR)技术检测抗氧化酶基因(SOD、POD、APX及CAT)表达量。结果表明，与CK组相比，Cd4组小白菜根中Cd含量、H2O2和MDA含量显著升高，而地下部鲜重、地上部鲜重、抗氧化酶(SOD、POD和APX)活性和叶绿素含量整体显著降低(P<0.05)。与Cd4组相比，Cd+PUE共处理组小白菜氧化损伤程度减轻，H2O2
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| 关 键 词： | 葛根素 镉损伤 小白菜 保护作用 |
| 收稿时间： | 2022-02-14 |
Protective Effect of Puerarin on Toxic Damage Caused by Cadmium in Pakchoi |
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| HU Yuanfeng,ZHANG Ming,FAN Shuying,GE Fei,ZHU Weifeng,XIAO Xufeng.Protective Effect of Puerarin on Toxic Damage Caused by Cadmium in Pakchoi[J].Acta Agriculturae Nucleatae Sinica,2023,37(2):424-431. |
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| Authors: | HU Yuanfeng ZHANG Ming FAN Shuying GE Fei ZHU Weifeng XIAO Xufeng |
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| Institution: | 1.College of Agronomy，Jiangxi Agricultural University，Nanchang，Jiangxi 3300452.Department of Animal Science，Jiangxi Biotech and Vocational College，Nanchang，Jiangxi 3302003.College of Pharmacy，Jiangxi University of Chinese Medicine，Nanchang，Jiangxi 330004 |
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| Abstract: | To investigate protective effects of puerarin （PUE） on cadmium （Cd） damage in pakchoi， different Puerarin （PUE） treatments with exogenous addition were taken， and the pakchoi were randomly divided into seven groups： control （CK）， PUE30 （puerarin 30 μmol·L-1）， Cd4 （Cd4 mg·L-1）， Cd and PUE co-treatment groups （4+15， 4+30， 4+45， 4+60）. After 30 d of treatment， the plant growth， Cd enrichment， stress-resistant physiological active substances including antioxidant enzymes （SOD， APX， CAT， POD） activities and hydrogen peroxide （H2O2）， malondialdehyde （MDA）， chlorophyll， Vc， soluble protein contents were observed， and the expression of antioxidant enzymes genes （Cu/Zn-SOD， POD， APX， and CAT） were measured by qRT-PCR. The results showed that the Cd enrichment， H2O2 and MDA contents in the roots of pakchoi were significantly higher in the Cd4 group compared with the CK group， while the root weight， fresh weight， antioxidant enzymes （SOD， POD， and APX） activities and chlorophyll content were significantly lower overall （P<0.05）. Compared with the Cd4 group， the Cd+PUE co-treatment group reduced oxidative damage， significantly decreased H2O2 and MDA contents， and significantly increased chlorophyll， Vc， and soluble protein contents as well as four antioxidant enzymes activities （P<0.05）； the appropriate concentration of PUE could alleviate the inhibition of Cd on the above-ground growth of pakchoi and reduce Cd uptake. In addition， the expression of Cu/Zn-SOD， POD， APX， and CAT genes in the Cd+PUE co-treatment group was higher than that in the Cd4 group， and the expression of Cu/Zn-SOD， POD， and CAT showed a increase followed by a decrease， while APX showed a decrease followed by an increase. In conclusion， the appropriate dose of Puerarin has a specific protective effect against oxidative damage under Cd stress in pakchoi. The results of this study provide a scientific basis for the application of gerbera in the prevention and control of Cd pollution and the improvement of vegetable yield. |
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| Keywords: | puerarin Cd damage pakchoi protective effect |
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