Assessing and broadening genetic diversity of a rapeseed germplasm collection |
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| Authors: | Jinfeng Wu Feng Li Kun Xu Guizhen Gao Biyun Chen Guixin Yan Nian Wang Jiangwei Qiao Jun Li Hao Li Tianyao Zhang Weiling Song Xiaoming Wu |
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| Institution: | Key Laboratory of Oil Crops Biology and Genetic Improvement, Ministry of Agriculture, Oil Crops Research Institute, CAAS, Wuhan 430062, China |
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| Abstract: | Assessing the level of genetic diversity within a germplasm collection contributes to evaluating the potential for its utilization as a gene pool to improve the performance of cultivars. In this study, 45 high-quality simple sequence repeat (SSR) markers were screened and used to estimate the genetic base of a world-wide collection of 248 rapeseed (Brassica napus) inbred lines. For the whole collection, the genetic diversity of A genome was higher than that of C genome. The genetic diversity of C genome for the semi-winter type was the lowest among the different germplasm types. Because B. oleracea is usually used to broaden the genetic diversity of C genome in rapeseed, we evaluated the potential of 25 wild B. oleracea lines. More allelic variations and a higher genetic diversity were observed in B. oleracea than in rapeseed. One B. oleracea line and one oilseed B. rapa line were used to generate a resynthesized Brassica napus line, which was then crossed with six semi-winter rapeseed cultivars to produce 7 F1 hybrids. Not only the allele introgression but also mutations were observed in the hybrids, resulting in significant improvement of the genetic base. |
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| Keywords: | genetic diversity allelic variation Brassica oleracea resynthesized Brassica napus SSR mutation |
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