| 不同生态区谷子品种表型鉴定及SSR遗传多样性分析 |
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| 引用本文： | 吕建珍,王宏勇,任莹,马建萍,赵凯.不同生态区谷子品种表型鉴定及SSR遗传多样性分析[J].核农学报,2023,37(3):471-482. |
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| 作者姓名： | 吕建珍 王宏勇 任莹 马建萍 赵凯 |
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| 作者单位： | 山西农业大学农学院，山西 太原 030031 |
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| 基金项目： | 山西省重点研发计划项目(201903D221077);财政部和农业农村部：国家现代农业产业技术体系资助(CARS-06-14.5-B8);山西省现代农业产业技术体系(GZTX202209);山西农业大学十四五生物育种工程项目(YZGC103) |
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| 摘 要： | 为了明确不同生态区谷子品种的遗传多样性和遗传结构，本研究对175份来源不同的谷子品种进行29个表型性状鉴定和36对简单序列重复（SSR）标记分析。结果表明，谷子数量性状的平均遗传多样性指数最高，质量性状最低；不同类性状中，单穗码数、开花至成熟日数和粒色遗传多样性指数最高，分别为2.082 8、2.012 5和1.078 7。36对SSR引物中，共检测到401个等位基因，平均11.4个，Shannon指数和多态性信息含量（PIC）平均为2.017 2和0.810 6；标记B142、B225的Shannon指数和PIC值最高，是评价谷子遗传多样性的理想SSR标记。不同生态区表型和SSR标记多样性比较结果表明，春谷中晚熟区（ML）遗传多样性指数最高，春谷早熟区（EM）次之，夏谷区（SS）最低；春谷中晚熟区谷子品种有10个性状指标最高，且全生育期最长。春谷中晚熟区不同类型材料中，汾阳的质量性状遗传多样性指数和SSR标记Shannon指数均最高，长治数量性状遗传多样性指数最高，太原Ⅱ的生育期遗传多样性指数最高；不同类型材料间差异较低，其中7个数量性状指标差异未达显著水平；汾阳的株高最高，出谷率最...
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| 关 键 词： | 谷子 生态区 表型鉴定 SSR标记 遗传分析 |
| 收稿时间： | 2022-05-18 |
Phenotypic Evaluation and SSR Genetic Diversity Analysis of Foxtail Millet Cultivars in Different Ecological Regions |
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| LYU Jianzhen,WANG Hongyong,REN Ying,MA Jianping,ZHAO Kai.Phenotypic Evaluation and SSR Genetic Diversity Analysis of Foxtail Millet Cultivars in Different Ecological Regions[J].Acta Agriculturae Nucleatae Sinica,2023,37(3):471-482. |
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| Authors: | LYU Jianzhen WANG Hongyong REN Ying MA Jianping ZHAO Kai |
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| Institution: | College of Agriculture，Shanxi Agricultural University，Shanxi，Taiyuan 030031 |
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| Abstract: | In order to identify the genetic diversity and population structure of foxtail millet cultivars from different ecological regions， 29 phenotypic traits and 36 pairs of SSR markers were texted with 175 foxtail millet cultivars. The results showed that the genetic diversity index of quantitative traits was the highest， and the qualitative traits was the lowest. The phenotypic traits with the highest genetic diversity indexes were spike number （2.082 8）， days from flowering to maturity （2.012 5） and grain color （1.078 7）. Alleles of 401 were detected by 36 pairs of SSR primers， with an average of 11.4 per alleles. The average Shannon index was 2.017 2， and polymorphic information contents （PIC） was 0.810 6. Markers B142 and B225 were found to be more suitable to evaluate the genetic similarity of foxtail millet. Phenotypic and SSR marker diversity comparison results showed that middle and late-maturing spring-sowing region （ML） had the highest diversity level， followed by early-maturing spring-sowing region （EM）， and summer-sowing region （SS） is the lowest. The 10 trait indexes of millet varieties in ML were the highest， and the whole growth period was the longest. In the ML， Fenyang had the highest phenotype and SSR marker diversity level， Changzhi had the highest genetic diversity index of quantitative traits， TaiyuanⅡ had the highest genetic diversity index of growth period. In different types of ML， the differences of 7 quantitative traits were not significant. Fenyang had the highest plant height and the lowest milled millet percentage， while Taiyuan had the longest panicle neck， but significantly lower panicle diameter. Clustering analysis based on both phenotypes and SSR markers was correlated with the origin of varieties. Cultivars derived from Jingu 21 or Yugu 18 were clustered when clustering with phenotypic traits， but became discretely when clustering by molecule marker. The study will provide scientific basis for genetic study and selection of new varieties of foxtail millet resources. |
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| Keywords: | foxtail millet ecological region phenotypic evaluation SSR maker genetic analysis |
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