| 茶树腐皮镰刀菌拮抗菌株的筛选鉴定及促生防病特性分析 |
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| 引用本文： | 邓晓旭,谢夏,潘娅梅,赵丰华,蒋双丰,徐文,张洁,孙润红,夏明聪,杨丽荣.茶树腐皮镰刀菌拮抗菌株的筛选鉴定及促生防病特性分析[J].茶叶科学,2023,43(1):67-77. |
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| 作者姓名： | 邓晓旭 谢夏 潘娅梅 赵丰华 蒋双丰 徐文 张洁 孙润红 夏明聪 杨丽荣 |
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| 作者单位： | 1.河南省农业科学院植物保护研究所/河南省生物农药工程研究中心,河南 郑州 450002;2.信阳市农业科学院,河南 信阳 464000 |
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| 基金项目： | 中央引导地方科技发展资金（YDZX20204100003141）、河南省农业科学院科技创新团队专项（2022TD25） |
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| 摘 要： | 腐皮镰刀菌（Fusariumsolani）是可以引起茶树茎腐和枯萎的致病真菌。为获得对茶树腐皮镰刀菌有拮抗效果的生防菌株，从河南省信阳市新县茶园收集的茶树（Camelliasinensis）健康叶片中采用梯度稀释法分离纯化得到内生细菌56株，通过平板对峙法筛选对腐皮镰刀菌抑制效果较好的菌株，并根据形态特征、生理生化特征和分子生物学技术对其进行鉴定，测定其防病促生指标并进行抑菌谱试验，通过发酵液试验和平板对扣法评估挥发性物质对腐皮镰刀菌菌丝生长的影响。结果显示，筛选得到的菌株YB-1476对腐皮镰刀菌菌丝生长抑制率达到63.31%，拮抗效果最佳，鉴定其为贝莱斯芽孢杆菌（Bacillus velezensis）。该菌株有产铁载体和吲哚乙酸、分泌β-1,3-葡聚糖酶和纤维素酶及溶磷的能力。抑菌谱试验表明，菌株YB-1476对平脐蠕孢菌（Bipolarissorokinana）、禾谷镰刀菌（FusariumgraminearumPH-1）、念珠状镰刀菌（Fusarium moniliforme）、尖孢镰刀菌（Fusariumoxysporumf.sp.niveum）和茄链格孢菌（Alterna...
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| 关 键 词： | 腐皮镰刀菌 茶树 茎腐和枯萎病 生物防治 |
| 收稿时间： | 2022-08-12 |
Screening and Identification of Strains against Fusarium solani Isolated from Camellia sinensis and Analysis of its Biocontrol and Growth Promotion Characteristics |
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| DENG Xiaoxu,XIE Xia,PAN Yamei,ZHAO Fenghua,JIANG Shuangfeng,XU Wen,ZHANG Jie,SUN Runhong,XIA Mingcong,YANG Lirong.Screening and Identification of Strains against Fusarium solani Isolated from Camellia sinensis and Analysis of its Biocontrol and Growth Promotion Characteristics[J].Journal of Tea Science,2023,43(1):67-77. |
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| Authors: | DENG Xiaoxu XIE Xia PAN Yamei ZHAO Fenghua JIANG Shuangfeng XU Wen ZHANG Jie SUN Runhong XIA Mingcong YANG Lirong |
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| Institution: | 1. Institute of Plant Protection Research, Henan Academy of Agricultural Sciences/Biological Pesticides Engineering Research Center of Henan Province, Zhengzhou 450002, China;2. Xinyang Academy of Agricultural Sciences, Xinyang 464000, China |
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| Abstract: | Fusarium solani is a pathogen that could cause collar canker and dieback of Camellia sinensis. To obtain strains with strong antagonistic activity against F. solani, a total of 56 endophytic strains were isolated and purified from healthy leaves of tea trees collected from a tea garden in Xinxian, Xinyang, Henan province by dilution plate method and were screened in dual cultures with F. solani. The strain was identified based on morphological characteristics, physiological, biochemical and molecular tests. Biocontrol and growth-promoting traits and antibacterial spectrum test were also detected. The effects on the mycelial growth of F. solani were evaluated by ferment product and volatile test by buckle culture. The results show that strain YB-1476 exhibited the strongest antagonistic activity against F. solani with growth inhibition rate of 63.31% and was identified as Bacillus velezensis. Strain YB-1476 could produce siderophore, indoleacetic acid, and be able to secrete β-1,3-glucanase, cellulase and solubilize phosphorus. Furthermore, strain YB-1476 displayed strong antagonistic activity against Bipolaris sorokinana, Fusarium graminearum PH-1, Fusarium moniliforme, Fusarium oxysporum f. sp. niveum and Alternaria solani and growth inhibition rates were 65.03%, 42.32%, 51.67%, 52.33% and 63.22%, respectively. In addition, ferment products show that the growth inhibition rates of the original fermentation broth, 10-fold, and 100-fold dilutions were 66.67%, 51.85%, 18.52%, respectively and the volatile test shows that growth inhibition rate against F. solani was 53.37%. The above results demonstrate that B. velezensis YB-1476 is a strain with great application potential to control collar canker and dieback of tea trees. |
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| Keywords: | Fusarium solani Camellia sinensis collar canker and dieback biocontrol |
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