| 坛紫菜贝壳丝状体白斑病附生细菌及藻际细菌群落特征分析 |
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| 引用本文： | 缪凡,刘鑫,林岗,陈燕婷,刘燕飞,郑怡.坛紫菜贝壳丝状体白斑病附生细菌及藻际细菌群落特征分析[J].中国农学通报,2023,39(5):154-164. |
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| 作者姓名： | 缪凡 刘鑫 林岗 陈燕婷 刘燕飞 郑怡 |
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| 作者单位： | 1. 福建师范大学生命科学学院;2. 福建省特色海洋生物资源可持续利用重点实验室;3. 福建省水产技术推广总站 |
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| 基金项目： | 2020年度福建省海洋与渔业保护及发展专项项目“海洋渔业重要经济物种资源监测”(022003); 中国农技协福建省科协建设项目“平潭坛紫菜科技小院” |
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| 摘 要： | 坛紫菜是最具经济价值的海产经济作物之一，贝壳丝状体白斑病严重影响坛紫菜育苗产业的健康发展。探究坛紫菜白斑病贝壳丝状体附生细菌群落和藻际细菌群落可为阐明白斑病的致病微生态机制提供一定的依据。本研究基于高通量测序技术分析了健康和患病的贝壳丝状体附生细菌、藻际细菌群落结构及对贝壳丝状体的影响。结果表明：白斑病贝壳丝状体上附生细菌丰度和多样性降低、藻际细菌丰度和多样性增加，健康池水样和患白斑病池水样的细菌群落结构有显著差异。健康池水样和患白斑病池水样中变形菌门Proteobacteria和拟杆菌门Bacteroidota为优势菌，水源水中蓝细菌门Cyanobacteria和变形菌门为优势菌；Nautella、Vibrio在患白斑病的贝壳丝状体水体中为优势菌；在白斑病贝壳丝状体附生细菌中土芽孢杆菌属Geobacillus丰度为18.25%，而健康贝壳丝状体仅为0.05%，差异极显著。培育池水体中高丰度的弧菌和贝壳丝状体上高丰度的土芽孢杆菌，都是坛紫菜贝壳丝状体白斑病的重要病因，在坛紫菜育苗期间藻际细菌及贝壳丝状体附生细菌群落结构的稳定是坛紫菜育苗的关键因素。
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| 关 键 词： | 坛紫菜 白斑病 细菌群落 高通量测序 贝壳丝状体 |
| 收稿时间： | 2022-07-07 |
Characteristics Analysis of Phycosphere Bacteria and Algal Bacterial Communities of Shell Conchocelis of Porphyrahaitanensis with White Spot Disease |
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| MIAO Fan,LIU Xin,LIN Gang,CHEN Yanting,LIU Yanfei,ZHENG Yi.Characteristics Analysis of Phycosphere Bacteria and Algal Bacterial Communities of Shell Conchocelis of Porphyrahaitanensis with White Spot Disease[J].Chinese Agricultural Science Bulletin,2023,39(5):154-164. |
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| Authors: | MIAO Fan LIU Xin LIN Gang CHEN Yanting LIU Yanfei ZHENG Yi |
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| Abstract: | Porphyrahaitanensis is one of the most valuable marine economic crops. The shell conchocelis white spot disease has seriously affected the healthy development of Porphyrahaitanensis seedling industry. The study on the epiphytic bacterial community of shell conchocelis and the bacterial community in the phycosphere of Porphyrahaitanensis white spot disease can provide a basis for elucidating the pathogenic microecological mechanism of white spot disease. Based on high-throughput sequencing technology, this study analyzed the community structure of healthy and diseased shell conchocelis epiphytic bacteria and phycosphere bacteria and their effects on shell conchocelis. The results showed that the abundance and diversity of epiphytic bacteria on the conchocelis of white spot shellfish decreased, and the abundance and diversity of bacteria in the phycosphere increased. There were significant differences in the bacterial community structure between the healthy pool water and the pool water with white spot disease. Proteobacteria and bacteroidota were the dominant bacteria in water samples from healthy pools and pools suffering from white spot disease, Cyanobacteria and Proteobacteria were the dominant bacteria in water source; Nautella and Vibrio were dominant bacteria in the shell conchocelis body water with white spot disease. Among the epiphytic bacteria of white spot shell conchocelis, the abundance of Geobacillus was 18.25%, while that of healthy shell conchocelis was only 0.05%, with a highly significant difference. The high abundance of Vibrio in the water body of the cultivation pond and the high abundance of Geobacillus on the shell conchocelis are both important causes of Porphyrahaitanensis shell conchocelis white spot disease. The stability of the community structure of phycosphere bacteria and shell conchocelis epiphytic bacteria is the key factor in the seedling raising of Porphyrahaitanensis. |
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| Keywords: | Porphyrahaitanensis white spot disease bacterial communities high-throughput sequencing shell conchocelis |
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