| 培矮64s为不育系的两系杂交稻杂种纯度的RAPD鉴定 |
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| 引用本文： | 杨蜀岚,伏健民,杨仁崔,王斌.培矮64s为不育系的两系杂交稻杂种纯度的RAPD鉴定[J].福建农林大学学报(自然科学版),1998(1). |
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| 作者姓名： | 杨蜀岚 伏健民 杨仁崔 王斌 |
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| 作者单位： | 福建农业大学作物科学学院，中国科学院遗传研究所 |
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| 摘 要： | 用２５０个Ｏｐｅｒｏｎ引物，对以培矮６４ｓ为母本配制的两系稻杂种及有关亲本等１２份供试材料进行ＲＡＰＤ分析，从中筛选出引物ＯＰＮ－２０．它不但能使培矮６４ｓ和以它为母本的杂种种子同其它种子加以区别，同时还扩增出恢复系山青、特青的多态性特征带，使它们的Ｆ１种子与母本培矮６４ｓ分开．并讨论了ＲＡＰＤ技术在两系杂交稻种子纯度鉴定上的应用
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| 关 键 词： | 两系杂交稻 培矮64s RAPD 杂种纯度鉴定 |
The purity identification of two line hybrids based on Peiai 64s with RAPD marker |
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| Yang Shulan,Fu Jianming,Yang Rencui,Wang Bin.The purity identification of two line hybrids based on Peiai 64s with RAPD marker[J].Journal of Fujian Agricultural and Forestry University,1998(1). |
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| Authors: | Yang Shulan Fu Jianming Yang Rencui Wang Bin |
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| Institution: | Yang Shulan 1 Fu Jianming 2 Yang Rencui 1 Wang Bin 2 |
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| Abstract: | The DNAs of 12 rice parental lines and F 1 hybrids and lines, relating Peiai 64s hybrid system, were detected with 250 Operon primers. The results based on RAPD analysis indicated that the primer OPN 20 could be used to distinguish Peiai 64s and its hybrids from the other materials. Futhermore, the primer OPN 20 could make restore lines, Shanqing and Teqing, amplify specific bands. The practical utilization of the RAPD marker technique in identifying the purity of two line hybrids of rice was also discussed. |
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| Keywords: | two line hybrids of rice Peiai 64s RAPD the purity identification |
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