| 丁吡吗啉对致病疫霉的作用机制初探 |
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| 引用本文： | 黄雄英,袁会珠,覃兆海,陈小霞,齐淑华.丁吡吗啉对致病疫霉的作用机制初探[J].农药学学报,2007,9(4):376-382. |
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| 作者姓名： | 黄雄英 袁会珠 覃兆海 陈小霞 齐淑华 |
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| 作者单位： | 1.中国农业科学院 植物保护研究所 农业部农药化学与应用技术重点开放实验室,北京 100094 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家科技支撑计划 |
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| 摘 要： | 丁吡吗啉(pyrimorph)是一种结构新颖的杀菌剂,室内生物测定结果表明,其对致病疫霉Phytophthora infestans的菌丝生长、孢子囊产生、休止孢萌发具有较强的抑制作用,其EC50值分别为0.066、0.059和0.550 μg/mL,但对游动孢子释放的抑制作用较弱,其EC50值为 9.78 μg/mL。显微镜下观察结果表明,经丁吡吗啉处理后的菌丝分枝相对较少,分枝间距拉长,但对 菌丝直径无影响;从致病疫霉对番茄叶片的侵染过程来看,丁吡吗啉浓度为100 μg/mL时,处理24 h后在叶片表面只能看到少量休止孢萌发,96 h后未产生孢子囊。丁吡吗啉在1 μg/mL和10 μg/mL 时对菌丝体细胞膜的电导率无影响,但当浓度为50 μg/mL时其电导率值明显上升;初步测定结果表明,在100 μg/mL浓度下,其对致病疫霉菌丝体的蛋白质合成有一定的抑制作用,但对其菌丝体内的DNA合成没有明显的抑制作用。
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| 关 键 词： | 丁吡吗啉 致病疫霉 生物活性 作用机制 |
| 文章编号： | 1008-7303（2007）04-0376-08 |
| 收稿时间： | 2007-05-10 |
| 修稿时间： | 2007-10-30 |
Preliminary Studies on the Mode of Action of Pyrimorph against Phytophthora infestans |
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| HUANG Xiong-ying,YUAN Hui-zhu,QIN Zhao-hai,CHEN Xiao-xia and QI Shu-hua.Preliminary Studies on the Mode of Action of Pyrimorph against Phytophthora infestans[J].Chinese Journal of Pesticide Science,2007,9(4):376-382. |
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| Authors: | HUANG Xiong-ying YUAN Hui-zhu QIN Zhao-hai CHEN Xiao-xia and QI Shu-hua |
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| Institution: | 1.Institute of Plant Protection,Chinese Academy of Agricultural Science,Beijing 100094,China2.Hunan Agricultural University,Changsha 410128,China3.Colloge of Science,China Agricultural University,Beijing 100094,China |
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| Abstract: | Pyrimorph is a novel fungicide developed by China Agricultural University and Institute of Plant Protection.Bioassay of pyrimorph to Phytophthora infestans was conducted.The results showed that the toxicity of pyrimorph was high in controlling P.infestans.It could intensively inhibit the mycelium growth,sporangium production and cystospore germination of P.infestans and the EC50 value was 0.066, 0.059 and 0.550 μg/mL, respectively.It was insufficient in inhibiting the release of zoospores from sporangia and the EC50 value was 9.78 μg/mL.Microscopic observation of pyrimorph treatment showed that the mycelium branch was fewer and the space was longer than that of CK.The difference of hypha diameter was not distinct between pyrimorph treatment and CK.When P.infestans invaded inside tomato leaves,it showed that only a few cystospores germinated 24 hours after inoculation and there was no sporangium 96 hours after inoculation of pyrimorph treatment at 100 μg/mL.Pyrimorph was found to influence on the permeability of the cell membrane of P.infestans at 50 μg/mL,and the difference was not distinct at 1 μg/mL and 10 μg/mL.It showed that pyrimorph had some effect on the protein biosynthesis of P.infestans at 100μg/mL,but had little effect on the DNA biosynthesis. |
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| Keywords: | pyrimorph Phytophthora infestans biological activity action mode |
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