| 采用分子生物学技术分析不同施肥土壤中细菌多样性 |
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| 引用本文： | 吴展才,余旭胜,徐源泰.采用分子生物学技术分析不同施肥土壤中细菌多样性[J].中国农业科学,2005,38(12):2474-2480. |
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| 作者姓名： | 吴展才 余旭胜 徐源泰 |
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| 作者单位： | 台湾大学园艺学研究所,台北,10600 |
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| 摘 要： | 本研究探讨在小白菜栽培时，分别添加果蔬加工废弃物生产的堆肥和化肥对土壤微生物区系的影响。利用扩增16S rRNA基因来比较微生物群落的技术，分离出所有群落DNA，再以此DNA为模板，以专一性引物扩增出16S rRNA基因（rDNA）。随后建立16S rDNA基因文库后，再以计算机仿真的方式，选出能将上述引物扩增的16S rDNA基因中间切割成片段长度范围集中的一组限制性内切酶--AciI、BstUI、和RsaI。结果表明，无施肥土壤与添加堆肥土壤中微生物较接近。无施肥、添加堆肥、添加化肥土壤三者的微生物多样性指数分别为0.990、0.986和0.962；均匀度为0.979、0.977和0.931。
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| 关 键 词： | 堆肥 化肥 土壤微生物 多样性 均匀度 |
| 收稿时间： | 09 14 2004 12:00AM |
| 修稿时间： | 2004-09-14 |
Analysis on Microbial Diversity of Different Agricultural Soils by Using Molecular Biology Technique |
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| NG Chang-chai,YU Hsu-sheng,SHYU Yuan-tay.Analysis on Microbial Diversity of Different Agricultural Soils by Using Molecular Biology Technique[J].Scientia Agricultura Sinica,2005,38(12):2474-2480. |
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| Authors: | NG Chang-chai YU Hsu-sheng SHYU Yuan-tay |
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| Institution: | Institute of Horticulture, Taiwan University, Taibei 10600 |
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| Abstract: | The microbial diversity and evenness in three different soil samples: compost-treated, chemical fertilizer-treated and control group soil samples were studied. The 16S rRNA gene cloning and restriction fragment length polymorphism (RFLP) were used for the comparison of soil microbe community. The optimization of RFLP digestion was firstly carried out, and AciI, BstUI, RsaI were optimal restriction enzymes for the digestion. The diversity indexes of soil microbes were also calculated and the indices of three treatments: control, compost, and chemical fertilizer-treated soils, are 0.990, 0.986, and 0.962 for D value, respectively; and 0.979, 0.977, and 0.931 for evenness, respectively. |
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| Keywords: | Compost Chemical fertilizer Soil microorganism Diversity Evenness |
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