| 云南鬼针草上发现鬼针草斑驳病毒 |
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| 引用本文： | 王建光,陈海如,苏云松,孔宝华,范静华.云南鬼针草上发现鬼针草斑驳病毒[J].中国农业科学,2009,42(5):1849-1853. |
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| 作者姓名： | 王建光 陈海如 苏云松 孔宝华 范静华 |
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| 作者单位： | 1. 农业生物多样性与病虫害控制教育部重点实验室,云南省植物病理重点实验室,云南农业大学,昆明,650201
2. 云南省玉溪市烟草公司,云南玉溪,650201 |
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| 摘 要： | 【目的】鉴定侵染鬼针草引起花叶或斑驳的病原。【方法】利用电镜技术观测病原粒子;间接ELISA方法鉴定病原与马铃薯Y病毒属病毒的血清学关系;分子生物学技术克隆了病原3′-端序列;BLAST，Clustal_X，BioEdit和MEGA 4.0等生物信息学软件用于所得序列的序列分析。【结果】在电镜下可观察到长约650-700 nm×13 nm左右的线状病毒粒子和风轮状内含体;利用Potyvirus PathoScren试剂盒检测呈阳性;用马铃薯Y病毒科病毒简并引物可扩增获得一条约1 800 bp的片段;序列分析表明该序列与鬼针草斑驳病毒相应序列高度相似，外壳蛋白的氨基酸同源性及3′-UTR核苷酸同源性均达96%以上。【结论】侵染云南鬼针草引起花叶或斑驳症状的病原为鬼针草斑驳病毒，这是该病毒侵染中国鬼针草属植物的首次报道。
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| 关 键 词： | 鬼针草 马铃薯Y病毒属 鬼针草斑驳病毒 |
| 收稿时间： | 2008-7-31 |
Natural Infection of Bidens pilosa L. by Bidens mottle virus in Yunnan |
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| WANG Jian-guang,CHEN Hai-ru,SU Yun-song,KONG Bao-hua,FAN Jing-hua.Natural Infection of Bidens pilosa L. by Bidens mottle virus in Yunnan[J].Scientia Agricultura Sinica,2009,42(5):1849-1853. |
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| Authors: | WANG Jian-guang CHEN Hai-ru SU Yun-song KONG Bao-hua FAN Jing-hua |
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| Institution: | Ministry of Education Key Laboratory of Agriculture Biodiverstiy for Plant Disease Management, The Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University |
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| Abstract: | 【Objective】 This study was carried out to determine the pathogen causing the mosaic and mottle symptom on Bidens pilosa L.. 【Method】 Viral particles in the infected leaves were detected by electron microscopy. Serological relation between the pathogenic virus and genus Potyvirus was examined by indirect ELISA. Moreover, the sequence of the 3′-part genome of the virus was cloned by RT-PCR and sequenced by common molecular biological methods. For bioinformational analysis, programs of BLAST at NCBI, Clustal_X, BioEdit and MEGA 4.0 were used for alignment and phylogenetic tree construction. 【Result】 Electron microscopy of leaf dips from infected plants revealed flexuous particles 650-700 nm in length and 13 nm in width. Cytoplasmic laminar aggregates and pinwheel inclusions were observed in ultrathin sections. Serological reaction between the virus and the antibody of potyvirus was positive. A DNA fragment of 1.8 kb with high identity to the sequence of Bidens mottle virus (BiMoV) could be amplified through the universal primers of family Potyviridae virus. Analysis of the sequence revealed that the amino acid identity of CP and nucleotide identity of 3′UTR to corresponding sequences of BiMoV were both more than 96%. 【Conclusion】 The evidences suggests that the pathogen causing mosaic and mottle symptom on Bidens pilosa L. is BiMoV, which is the first report of BiMoV infecting a member of the Bidens L. in China. |
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| Keywords: | Bidens pilosa L genus Potyvirus Bidens mottle virus |
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