| 转BADH基因苜蓿T1代遗传稳定性和抗盐性研究 |
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| 引用本文： | 燕丽萍,夏阳,梁慧敏,王珍珍,李双云,刘翠兰,庞彩红.转BADH基因苜蓿T1代遗传稳定性和抗盐性研究[J].草业学报,2009,18(6):65-71. |
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| 作者姓名： | 燕丽萍 夏阳 梁慧敏 王珍珍 李双云 刘翠兰 庞彩红 |
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| 作者单位： | 1.山东省林业科学研究院,山东 济南 250014;2.江苏农林职业技术学院,江苏 句容 212400 |
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| 摘 要： | 采用分子生物学检测和不同浓度的NaCl对转BADH基因苜蓿T1代2个株系进行遗传稳定性和抗盐性研究。结果表明,2个株系的T1代PCR阳性植株和阴性植株分离比例都符合孟德尔遗传规律;在0.8%NaCl胁迫下,转基因植株Northern杂交表达量明显增加,BADH酶活性和甜菜碱含量分别比对照植株增加2～4和4～5倍;不同浓度NaCl胁迫下转基因株系脯氨酸和可溶性糖含量、抗氧化酶活性都显著高于对照植株,而电导率和丙二醛含量显著低于对照植株。表明转入的BADH基因可以稳定遗传,转基因苜蓿植株耐盐性明显高于对照。
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| 关 键 词： | 苜蓿 BADH基因 盐胁迫 |
| 收稿时间： | 1900-01-01; |
A study on salt tolerance and the genetic stability of T1 generation transgenic alfalfa with the BADH gene |
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| YAN Li-ping,XIA Yang,LIANG Hui-min,WANG Zhen-zhen,LI Shuang-yun,LIU Cui-lan,PANG Cai-hong.A study on salt tolerance and the genetic stability of T1 generation transgenic alfalfa with the BADH gene[J].Acta Prataculturae Sinica,2009,18(6):65-71. |
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| Authors: | YAN Li-ping XIA Yang LIANG Hui-min WANG Zhen-zhen LI Shuang-yun LIU Cui-lan PANG Cai-hong |
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| Institution: | 1.Shandong Provincial Academy of Forestry, Jinan 250014, China； 2.Jiangsu Agriculture and Forestry Profession Technology College, Jurong 212400, China |
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| Abstract: | The physiological response of transgenic alfalfa to different salt stresses and its PCR detection were investigated.The ratio of negative to positive numbers of PCR detection confirmed the first Mendelian law.The transgenenic T1 generations showed enhanced BADH activity and betaine content,compared to the non-transgenenic plants at 0.8% NaCl stress.Proline and soluble sugar contents and the antioxidase activity of transgenic T1 generations were higher than those of non-transgenic plants at different salt st... |
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| Keywords: | alfalfa BADH gene salt stress |
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