| 紫外线、He-Ne激光对葡萄白腐病菌拮抗菌PT2的复合诱变效应 |
| |
| 引用本文： | 巩洁,陈亮,陈立,韩文霞,陈五岭.紫外线、He-Ne激光对葡萄白腐病菌拮抗菌PT2的复合诱变效应[J].植物保护,2010,36(5):105-109. |
| |
| 作者姓名： | 巩洁 陈亮 陈立 韩文霞 陈五岭 |
| |
| 作者单位： | 西北大学生命科学学院,西安,710069 |
| |
| 摘 要： | 采用紫外线、He-Ne激光及两者复合诱变的方法,分别处理对葡萄白腐病有拮抗作用的枯草芽胞杆菌PT2,以提高其抑菌效果。结果表明,紫外线和He-Ne激光单独作用,所筛菌株UV10与HN14抑菌效果提高率分别为15.0%与21.7%。而经过对复合诱变条件确定,在紫外线照射90 s后,再用15 mW的He-Ne激光辐照10 min,筛选出最佳正变菌株F15,抑菌效果提高30.9%,明显高于单因素诱变,经25代传代试验证明其拮抗能力可以进行稳定遗传。
|
| 关 键 词： | 紫外线 He-Ne激光 枯草芽胞杆菌 葡萄白腐病菌 复合诱变 |
Mutagenetic effects of UV and He-Ne laser on the strain PT2 with antagonistic ability against Coniella diplodiella |
| |
| Gong Jie,Chen Liang,Chen Li,Han Wenxi,Chen Wuling.Mutagenetic effects of UV and He-Ne laser on the strain PT2 with antagonistic ability against Coniella diplodiella[J].Plant Protection,2010,36(5):105-109. |
| |
| Authors: | Gong Jie Chen Liang Chen Li Han Wenxi Chen Wuling |
| |
| Institution: | Institute of Life Sciences, Northwest University, Xi’an 710069, China |
| |
| Abstract: | The strain PT2 Bacillus subtilis, which has an antagonistic ability to Coniella diplodiella Petrak &; Syd. was irradiated respectively by UV and He-Ne laser, and the mutant strains UV10 and HN14 were obtained. Both of them increased the antagonistic ability by 15.0% and 21.7% compared with PT2. The results indicated that the coordinative effect of UV and He-Ne laser was better than that by using them individually. The cells were treated firstly by UV for 90 seconds and then by He-Ne laser for 10 minutes in 15mW. The best positive mutation strain F15 was obtained, whose antagonistic ability was 30.9% higher than PT2’s. By cultivating it from 1st to 25th generation, its antagonistic ability proved to be stable in heredity. |
| |
| Keywords: | UV He-Ne laser Bacillus subtilis Coniella diplodiella compound mutation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《植物保护》浏览原始摘要信息 |
| 点击此处可从《植物保护》下载免费的PDF全文 |
|
