| 肉苁蓉多糖提取工艺及抑菌作用研究 |
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| 引用本文： | 王晓琴,曹礼,朱艳萍.肉苁蓉多糖提取工艺及抑菌作用研究[J].安徽农业科学,2009,37(32):15855-15856. |
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| 作者姓名： | 王晓琴 曹礼 朱艳萍 |
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| 作者单位： | 王晓琴,曹礼(河西学院生命科学与工程系,甘肃张掖,734000;河西学院生态研究所,甘肃张掖,734000);朱艳萍(河西学院生命科学与工程系,甘肃张掖,734000) |
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| 摘 要： | 目的]提取肉苁蓉多糖并测定其抑菌作用。方法]采用热水浸提法提取肉苁蓉多糖，在单因素试验的基础上，通过3因素3水平正交试验优化肉苁蓉多糖提取工艺。结果]各因素对多糖得率的影响依次为浸提温度〉水料比〉浸提时间。最佳提取工艺为以1：15的料水比在85℃下浸提2h，利用该工艺提取肉苁蓉多糖的平均得率为6．7857mg／g。肉苁蓉多糖溶液对四叠球菌的抑制作用最强，其抑菌圈直径为11．20mm，其次为枯草芽孢杆菌，其抑菌圈直径为10．24mm。肉苁蓉多糖溶液对啤酒酵母的抑制作用较强，其抑菌圈直径为8．16mm，对黑曲霉和米曲霉的抑菌作用不明显。肉苁蓉多糖溶液对大肠杆菌、四叠球菌、枯草杆菌、啤酒酵母和橘青霉的最小抑菌浓度分别为0．437、0．109、0．218、0．437和0．874mg／ml。结论J该研究为肉苁蓉在更多领域中的合理开发和利用奠定了基础。
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| 关 键 词： | 肉苁蓉 多糖 提取工艺 抑菌作用 最小抑菌浓度 |
Study on the Extraction Technology and Antimicrobial Action of Cistanche deserticola Polysaccharides |
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| WANG Xiao-qin et al.Study on the Extraction Technology and Antimicrobial Action of Cistanche deserticola Polysaccharides[J].Journal of Anhui Agricultural Sciences,2009,37(32):15855-15856. |
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| Authors: | WANG Xiao-qin |
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| Institution: | WANG Xiao-qin et al (Department of Life Science , Engineering,Hexi University,Zhangye,Gansu 734000) |
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| Abstract: | Objective]The purpose was to extract Cistanche deserticola polysaccharides and determine their antimicrobial action.Method] The polysaccharides were extracted from C.deserticola by hot water extraction.On the basis of single factor experiments,the extraction technology was optimized through 3-factor and 3-level orthogonal test.Result]The influences of various factors on the polysaccharide yield were as follows:extraction temperature > ratio of water to material > extraction time.The optimum extraction te... |
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| Keywords: | Cistanche deserticola Polysaccharides Extraction technology Antimicrobial action Minimum inhibitory concentration |
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