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| 猪各组织器官内肌生成抑制素基因mRNA表达谱的研究 | |
| 引用本文： | 张锐,孙美榕,张红莲,杨捷,黄燕,陈绍红,杜慧,欧阳红生.猪各组织器官内肌生成抑制素基因mRNA表达谱的研究[J].中国兽药杂志,2004,38(3):16-18. |
| 作者姓名： | 张锐 孙美榕 张红莲 杨捷 黄燕 陈绍红 杜慧 欧阳红生 |
| 作者单位： | 1. 湛江海洋大学现代生化中心,广东湛江,524088 2. 深圳科安信公司,广东深圳,518057 3. 中国人民解放军军需大学生物化学与分子生物学教研室,吉林长春,130062 |
| 摘 要： | 用Trizol试剂法提取军牧一号猪的骨骼肌、心肌、肝、肾、肺、脾、胰、脂肪的总RNA做RT-PCR和嵌套PCR,结果在军牧一号猪骨骼肌、心肌和脂肪组织内检测到了肌生成抑制素mRNA的表达,而在其他组织中未检测到. |
| 关 键 词： | 猪 肌生成抑制素基因 表达谱 |
| 文章编号： | 1002-1280(2004)03-0016-03 |
| 修稿时间： | 2003年7月21日 |
Studies on the expression pattern of myostatin mRNA in porcine tissues |
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| ZHANG Rui,SUN Mei-rong,ZHANG Hong-lian,YANG Jie,HUANG Yan,CHEN Shao-hong,DU Hui,OUYANG Hong-sheng.Studies on the expression pattern of myostatin mRNA in porcine tissues[J].Chinese Journal of Veterinary Drug,2004,38(3):16-18. | |
| Authors: | ZHANG Rui SUN Mei-rong ZHANG Hong-lian YANG Jie HUANG Yan CHEN Shao-hong DU Hui OUYANG Hong-sheng |
| Institution: | ZHANG Rui~1,SUN Mei-rong~2,ZHANG Hong-lian~1,YANG Jie~1,HUANG Yan~1,CHEN Shao-hong~1,DU Hui~1,OUYANG Hong-sheng~3 |
| Abstract: | Myostatin is a negative regulator of muscle growth. It can be expressed in skeletal muscle, galactophore and cardiomyocytes. In order to investgate the expression pattern of myostatin mRNA in others tissues, total RNAs were isolated from skeletal muscle, cardiac muscle, liver, kidney, lung, spleen, pancreas, and fat cells of a new pig breed JunMu-1 with Trizol reagent. Using RT-PCR and a nested PCR, it was found that the expression of mRNAs were detected in skeletal muscle, cardiac muscle and fat, and not detected in the other tissues. The result was coincident with the published literatures. |
| Keywords: | mRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |