| 白三叶遗传转化体系的探讨 |
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| 引用本文： | 张边江,陈全战,周泉澄,华春.白三叶遗传转化体系的探讨[J].西南农业学报,2011,24(3). |
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| 作者姓名： | 张边江 陈全战 周泉澄 华春 |
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| 作者单位： | 南京晓庄学院，江苏南京，211171 |
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| 基金项目： | 南京晓庄学院生态学重点学科项目(2005NXY01); 江苏省教育厅高校自然基金项目(08KJD180012) |
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| 摘 要： | 将白三叶Trifolium repens L.无菌种子吸胀15 h后,制备子叶分别放在含不同浓度配比激素的培养基上培养,同时测定白三叶子叶对卡那霉素(Kam)、头孢霉素(Cef)和羧苄青霉素(Carb)的敏感性,探索出适合子叶生长成苗的一套比较完整的体系。结果表明,用于白三叶子叶遗传转化的培养基为MS+30 g/L蔗糖+8 g/L琼脂+0.1 mg/L NAA+0.5 mg/L6-BA+20 mg/L Kam+250 mg/L Carb。抗性植株最佳生根培养基为MS+15 g/L蔗糖+8 g/L琼脂+0.2 mg/L NAA。通过本实验成功确立了白三叶遗传转化体系,为下一步转基因实验奠定了基础。
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| 关 键 词： | 白三叶(Trifolium repens L.) 子叶 遗传转化 |
Discussion on Genetic Transformation System of White Clover(Trifolium repens L.) |
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| ZHANG Bian-jiang,CHEN Quan-zhan,ZHOU Quan-cheng,HUA Chun.Discussion on Genetic Transformation System of White Clover(Trifolium repens L.)[J].Southwest China Journal of Agricultural Sciences,2011,24(3). |
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| Authors: | ZHANG Bian-jiang CHEN Quan-zhan ZHOU Quan-cheng HUA Chun |
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| Institution: | ZHANG Bian-jiang,CHEN Quan-zhan,ZHOU Quan-cheng,HUA Chun(Nanjing Xiaozhuang College,Jiangsu Nanjing 211171,China) |
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| Abstract: | The sterile seeds of white clover were imbibited for 15 h to obtain the cotyledons that were cultured on different kinds of mediums with different concentrations of hormones.The sensitivity of cotyledons to Kam,Cefotaxime sodium and Carb was detected,respectively.The results showed that the optimum medium of genetic transformation contained MS+30 g/L sucrose+8 g/L agar+0.1 mg/L NAA+0.5 mg/L 6-BA +20 mg/L Kam+250 mg/L Carb.The optimum rooting medium was established(MS+15g/L sucrose+8g/L agar + 0.2 mg/L NAA).... |
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| Keywords: | White clover(Trifolium repens L) Cotyledon Genetic transformation |
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