| 豹纹鳃棘鲈消化道粘液细胞的类型与分布 |
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| 引用本文： | 王永波,张杰,李向民.豹纹鳃棘鲈消化道粘液细胞的类型与分布[J].海洋渔业,2016,38(5):478-486. |
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| 作者姓名： | 王永波 张杰 李向民 |
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| 作者单位： | 海南省海洋与渔业科学院,海口,571203 |
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| 基金项目： | 海南省科学事业费项目(KYYS －2013－18；KYYS －2014－56)；海洋公益性行业科研专项(201205028)；海南省重大科技项目(ZDZX2013014) |
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| 摘 要： | 应用AB-PAS染色法对工厂化养殖条件下豹纹鳃棘鲈(Plectropomus leopardus)消化道粘液细胞的类型与分布进行了观察研究。结果显示:豹纹鳃棘鲈粘液细胞在消化道各部位均有分布,不同部位的粘液细胞密度和类型均有差异,豹纹鳃棘鲈消化道粘液细胞可分为Ⅰ型、Ⅱ型、Ⅲ型、Ⅳ型。其中,食道粘液细胞以Ⅲ型为主,胃以Ⅰ型为主,幽门盲囊和肠以Ⅲ型为主,直肠以Ⅲ型和Ⅳ型为主;胃部粘液细胞分布密度最大,达(111.37±21.74)cell·mm~(-2),极显著高于消化道其它部分,直肠、幽门盲囊、肠的粘液细胞分布密度分别为(35.09±8.74)、(30.23±5.37)、(28.00±4.67)cell·mm~(-2),相互之间差异不显著,食道的粘液细胞分布密度最低,为(10.83±1.94)cell·mm~(-2),与消化道其它部分相比差异极显著。另外,消化道各段单位面积内粘液细胞的分泌能力顺序为食道直肠肠幽门盲囊胃。通过对消化道各部位粘液细胞的分类对比可以发现,粘液细胞的类型和分布与其所在部位的功能有密切关系。
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| 关 键 词： | 豹纹鳃棘鲈 消化道 粘液细胞 |
Types and distribution of mucous cells in the digestive tract of Plectropomus leopardus |
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| Abstract: | Plectropomus leopardus is a famous commercial fish species.A complete industrial chain,including cultivation,breeding,sales,transportation,has formed,and the economic benefit is remarkable in Hainan, Shandong and other provinces of China.To carry out the research on mucous cells in the digestive tract will help us understand the basic fish growth and development physiological mechanisms,and also has the vital significance in mariculture and disease control of Plectropomus leopardus.In this paper,the types and distribution of the digestive tract mucous cells of Plectropomus leopardus under factory farming conditions were observed.In October 2014,Plectropomus leopardus were selected from Qionghai scientific research base of Hainan Academy of Ocean and Fisheries Sciences for the observational study on the fish digestive tract mucous cell types and distribution by using Alcian blue-periodic acid sthiff (AB-PAS).The result shows that mucous cells are distributed in each part of the digestive tract,with different densities and types.Mucous cells in the digestive tract can be divided into type Ⅰ,type Ⅱ,type Ⅲ,and type Ⅳ.Among them,the mucous cells of esophagus are mainly type Ⅲ;stomach are mainly typeⅠ;pyloric caeca and intestine are mainly type Ⅲ;rectum are mainly type Ⅲ and Ⅳ.Mucous cells distribution density in stomach (111.37 ±21.74 cell· mm -2 )is the largest and significantly higher than that of other parts in the digestive tract;there is no significant difference among the distribution density in rectum (35.09 ±8.74 cell·mm -2 ),pyloric caeca (30.23 ±5.37 cell·mm -2 ),and intestine (28.00 ±4.67 cell·mm -2 );the density in esophagus (10.83 ±1.94 cell·mm -2 )is the lowest and significantly different from that of other parts in the digestive tract.For the secretion capacity of mucous cells per unit area in different parts of digestive tract of Plectropomus leopardus,esophagus and rectum is higher with P values of 3485.56 ±644.21 and 3044.84 ±781.01 respectively,the secretion capacity of esophagus is the highest and significantly different from that of rectum, and extremely significantly different from other parts in the digestive tract;there is no significant difference among the secretion capacity of intestine (1333.68 ±245.02),pyloric caeca (1326.51 ±237.08)and stomach (237.08 ±217.33).The distribution characteristics and features of the digestive tract mucous cells in different parts of the digestive tract of Plectropomus leopardus are almost completely consistent with that of carnivorous reef fish,adapted to the digestive physiology characteristic of the fish. |
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| Keywords: | Plectropomus leopardus digestive tract mucous cells |
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