| 两种杂交石斑鱼子一代及其亲本的线粒体COⅠ基因遗传变异分析 |
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| 引用本文： | 周翰林,杨森,高川,张磊,张海发,李水生,张勇,蒙子宁,刘晓春,林浩然.两种杂交石斑鱼子一代及其亲本的线粒体COⅠ基因遗传变异分析[J].热带生物学报,2012,3(1):1-10. |
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| 作者姓名： | 周翰林 杨森 高川 张磊 张海发 李水生 张勇 蒙子宁 刘晓春 林浩然 |
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| 作者单位： | [1]中山大学生命科学学院,广东广州510275 [2]广东省大亚湾水产试验中心,广东惠州516081 [3]海南大学海洋学院,海南海口570228 |
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| 基金项目： | 公益性行业（农业）科研专项（200903046）;国家自然基金面上项目（30871914）;广东省科技计划项目（20088020800010,20098020308001,20108090400551,20118020307009）,广东省海洋渔业科技推广专项（A200800H01,A200900H01） |
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| 摘 要： | 对两种杂交石斑鱼子一代（青龙斑和虎龙斑）及其亲本（斜带石斑鱼、棕点石斑鱼和鞍带石斑鱼）的线粒体DNA细胞色素氧化酶Ⅰ（COⅠ）基因序列进行了测序分析。在15个样本中，同源序列片段（1551bp）中共检测到9个单倍型和249个核苷酸多态位点。序列差异分析和遗传距离比较结果显示，核苷酸序列同源性在88．1％-100％之间，无明显遗传分化。虎龙斑的2个COⅠ基因单倍型与母本棕点石斑鱼单倍型的同源性为99％和100％，而与父本鞍带石斑鱼的同源性均为88．1％。青龙斑的2个单倍型与母本斜带石斑鱼的3个单倍型的同源性在99．7％-100％之间，而与父本鞍带石斑鱼的同源性分别为89．3％和89．4％，结果表明两种杂交子一代在线粒体DNACOⅠ基因上严格遵循母性遗传规律。
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| 关 键 词： | 斜带石斑鱼 鞍带石斑鱼 棕点石斑鱼 杂交子代 COⅠ 遗传变异 |
Analysis of Genetic Variability of mtDNA CO I Genes between two grouper hybrids and their parents |
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| ZHOU Han-lin,YANG Sen,GAO Chuan,ZHANG Lei,ZHANG Hai-fa,LI Shui-sheng,ZHANG Yong,MENG Zi-ning,LIU Xiao-chun,LIN Hao-ran.Analysis of Genetic Variability of mtDNA CO I Genes between two grouper hybrids and their parents[J].Journal of Tropical Biology,2012,3(1):1-10. |
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| Authors: | ZHOU Han-lin YANG Sen GAO Chuan ZHANG Lei ZHANG Hai-fa LI Shui-sheng ZHANG Yong MENG Zi-ning LIU Xiao-chun LIN Hao-ran |
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| Institution: | 1. College of Lite Sciences/Institute of Aquatic Economic Animals, Sun Yat - Sen (Zhongshan) University, Guangzhou 510275, China; 2. Guangdong Daya Bay Fisheries Development Center, Huizhou 516081 , China; 3. College of Ocean, Hainan University, Haikou 570228, China. |
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| Abstract: | In order to identify the phylogenetic relationship among two grouper hybrids （ Qinglong grouper and Hulong grouper） and their parents （ Epinephelus coioides 9, Epinephelus coioides ♀ and E. lanceolatus ♂ ）, their mitochondrial cytochrome oxidase subunit I （CO I ） gene fragments were amplified by PCR, and the 1551 base-pair nucleotide sequences of CO I were examined. The results show that all 15 sequences are grouped into 9 haplotypes and 249 nucleotide polymorphic loci in CO I gene fragments. Sequence divergences and genetic distance analysis showed all the groupers shared 88.1% to 100% similarities in nucleotide sequence, indicating there is no significant genetic difference among these groupers. The two CO I haplotypes of Hulong grouper showed 99% and 100% similarities with the haplotype of the female parent E. fuscoguttatus, respectively, but only 88.1% similarity with the male parent E. lanceolatus. The two CO I haplotypes in Qinglong grouper had similarities of 99.7% to 100% with the three haplotypes of the female parent E. coioides, and only of 89.3% and 89.4% with the male parent E. lanceolatus. The results indicated that the two grouper hybrids were inherited from their maternal parent in mitochondrial CO I genes. |
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| Keywords: | Epinephelus coioides E lanceolatus Qinglong grouper Hulong grouper CO I genetic variability |
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