|
|||||
|
|
| 甲型H1N1流感病毒血凝素基因的原核表达及用于胶体金检测方法的研究 | |
| 引用本文： | 王云龙,郭文丽,孙新城,李玉林,李恒思,陈冬焕,王真,董彩文,刘旺根.甲型H1N1流感病毒血凝素基因的原核表达及用于胶体金检测方法的研究[J].动物医学进展,2011,32(7):1-4. |
| 作者姓名： | 王云龙 郭文丽 孙新城 李玉林 李恒思 陈冬焕 王真 董彩文 刘旺根 |
| 作者单位： | 1. 河南师范大学,河南新乡453007;郑州职业技术学院,河南郑州450121 2. 河南师范大学,河南新乡,453007 3. 郑州大学,河南郑州,450001 4. 河南省生物工程技术研究中心,河南郑州,450001 |
| 基金项目： | 河南省工程技术中心专项经费项目(102102313105); 郑州市重大科技专项计划(093SGBS22027) |
| 摘 要： | 根据GenBank公布的甲型H1N1流感病毒血凝素(HA)基因序列,合成HA全长基因,构建原核表达载体pET-30 Xa/LIC-HA.阳性质粒转化E.coli BL21(DE3),IPTG诱导表达.对表达蛋白进行SDS-PAGE和Western blot检测的结果表明,HA基因获得表达,产物具有免疫学活性,分子质量约... |
| 关 键 词： | 甲型H1N1流感病毒 血凝素 原核表达 胶体金 |
Prokaryotic Expression of Hemagglutinin Gene of H1N1 Influenza A Virus and Its Preliminary Application in Colloidal Gold Immunochromatographic Assay |
|
| WANG Yun-long,GUO Wen-li,SUN Xin-cheng,LI Yu-lin,LI Heng-si,CHEN Dong-huan,WANG Zhen,DONG Cai-wen,LIU Wang-gen.Prokaryotic Expression of Hemagglutinin Gene of H1N1 Influenza A Virus and Its Preliminary Application in Colloidal Gold Immunochromatographic Assay[J].Progress In Veterinary Medicine,2011,32(7):1-4. | |
| Authors: | WANG Yun-long GUO Wen-li SUN Xin-cheng LI Yu-lin LI Heng-si CHEN Dong-huan WANG Zhen DONG Cai-wen LIU Wang-gen |
| Institution: | WANG Yun-long1,2,GUO Wen-li1,SUN Xin-cheng4,LI Yu-lin3,LI Heng-si3,CHEN Dong-huan3,WANG Zhen3,DONG Cai-wen3,LIU Wang-gen3(1.Henan Normal University,Xinxiang,Henan,453007,China,2.Zhengzhou Technical College,Zhengzhou,450121,3.Henan Bioengineering Research Center,450001,4.Zhengzhou University,China) |
| Abstract: | According to the relevant nucleotide sequence from GenBank,the hemagglutinin(HA) gene of H1N1 influenza A virus was synthetized.The product was used for constructing a prokaryotic recombinant plasmid pET-30 Xa/LIC-HA.The constructed vector was transformed to E.coli BL21(DE3) for expression under the induction of IPTG.The expressed product was identified by SDS-PAGE and Western blot.The target protein in the form of inclusion bodies,with a relative molecular mass of about 6 400,showed immunogenicity.A GICA f... |
| Keywords: | H1N1 influenza A virus hemagglutinin prokaryotic expression GICA |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |