| 猪圆环病毒2型GD株ORF2基因的序列分析 |
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| 引用本文： | 宋长绪,赵亚华,蒋智勇,王贵平,高向阳.猪圆环病毒2型GD株ORF2基因的序列分析[J].中国兽医杂志,2005,41(8):6-9. |
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| 作者姓名： | 宋长绪 赵亚华 蒋智勇 王贵平 高向阳 |
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| 作者单位： | [1]广东省农业科学院兽医研究所,广东广州510640 [2]华南农业大学生命科学院,广东广州510642 |
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| 基金项目： | 国家863计划资助项目（2003AA241110）;广东省农业攻关项目（2003821404） |
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| 摘 要： | 根据GenBank中猪圆环病毒2型（PCV2）0RF2基因序列，设计一对引物，应用PCR从本室鉴定分离的PCV2 GD株的细胞培养物中扩增出ORF2基因（702bp）。将此基因片段克隆入pMD18-T载体，筛选获得重组质粒pMD—ORF2并对其测序，结果表明所克隆的ORF2基因与其他PCV2的0RF2基因核苷酸序列同源性在92．1％～99．9％之间，推导的氨基酸序列同源性在90．2％～99．5％之间。
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| 关 键 词： | 猪圆环病毒2型（PCV2） ORF2基因 序列分析 猪圆环病毒2型 GD GenBank 序列同源性 基区 基因序列 PCV2 |
| 文章编号： | 0529-6005（2005）08-0006-04 |
| 收稿时间： | 01 5 2004 12:00AM |
| 修稿时间： | 2004年1月5日 |
Cloning and sequence analysis of ORF2 gene of porcine circovirus type 2 |
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| Song ChangXu;Zhao YaHua;Jiang ZhiYong;Wang GuiPing;Gao XiangYang.Cloning and sequence analysis of ORF2 gene of porcine circovirus type 2[J].Chinese Journal of Veterinary Medicine,2005,41(8):6-9. |
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| Authors: | Song ChangXu;Zhao YaHua;Jiang ZhiYong;Wang GuiPing;Gao XiangYang |
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| Abstract: | According to the published ORF2 genes sequence of PCV2 , two pairs of PCR primers were designed, and ORF2 genes was amplified from PCV2 GD strain isolated by our laboratory frome Guangdong. Then the PCR products was cloned into pMD 18-T easy vector, and the recombinant plasmids named pMD-ORF2 were obtained. The sequences of the cloned PCR products was analyzed and compared with other PCV2 isolates in GenBank, the results indicated that the homology of their nucleotide sequence was 92.1% to 99.9%, and the ORF2 gene displayed 93.4% to 99.7% identity based on amino acids with other PCV2 strains. |
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| Keywords: | porcine eircovirus type 2 ORF2 gene sequence analysis |
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