| 猪鸡组织中左旋咪唑残留的HPLC检测方法研究 |
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| 引用本文： | 曾勇.猪鸡组织中左旋咪唑残留的HPLC检测方法研究[J].中国兽药杂志,2010,44(8):17-21. |
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| 作者姓名： | 曾勇 |
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| 作者单位： | 湖北省兽药监察所,武汉,430070 |
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| 摘 要： | 建立了猪、鸡组织中左旋咪唑残留检测的高效液相色谱法。组织试样在碱性条件下先用乙酸乙酯提取,然后用0.1 mol/L盐酸反萃取,萃取的样液用混合型阳离子固相萃取小柱净化,采用C18(5μm,250 mm×4.6 mm i.d.)色谱柱,用高效液相色谱仪(带紫外检测器)在220 nm波长处测定,外标法定量。在此条件下,左旋咪唑的保留时间为12～14 min。左旋咪唑浓度在10～1 000μg/L时,与峰面积呈良好的线性关系,线性方程为y=196.37x+81.599(r=0.999 9)。结果表明,左旋咪唑在在猪、鸡的肌肉、脂肪和肾脏中的检测限为2μg/kg,定量限为5μg/kg;在猪、鸡的肝脏中的检测限为2μg/kg,定量限为10μg/kg。在添加不同浓度水平测定下,平均回收率在70%～110%,批内变异系数在10%以内,批间变异系数在15%以内。
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| 关 键 词： | 左旋咪唑 残留 高效液相色谱 |
| 收稿时间： | 2010/3/10 0:00:00 |
| 修稿时间： | 2010/4/22 0:00:00 |
Study on Levamisole Residues in Pig and Chicken Tissues by HPLC Method |
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| zengyong.Study on Levamisole Residues in Pig and Chicken Tissues by HPLC Method[J].Chinese Journal of Veterinary Drug,2010,44(8):17-21. |
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| Authors: | zengyong |
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| Institution: | Hubei Institute of Veterinary Drug Control |
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| Abstract: | High-performance liquid chromatography method was optimised and validated for the determination of levamisole in pig and chicken tissues.A liquid-liquid extraction procedure in alkaline medium,using ethyl acetate as extraction solvent.The ethyl acetate extracts were re-extracted with 0.1 mol/L HCl,then the solution was followed by a solid-phase extraction procedure using an MCX column to clean up.The reconstituted sample was transferred to HPLC system to detect,using the C18 column（5 μm,250 mm×4.6 mm i.d.）.Retention time of levamisole was 12～14 min.Linearity of the detector response was verified with robenidine standard solution in the range of 10～1 000 μg/L,the linear regression was y=196.37x＋81.599（r=0.999 9）.The quantitative methed indicated that,the LOD was 2 μg/kg and the LOQ was 5 μg/kg in Pigs,chicken muscle,fat and kidney,the LOD was 2 μg/kg and the LOQ was 10 μg/kg in Pigs,chicken liver.Recoveries of robenidine from spiked samples at different concentrations was between 70%～110%,the RSD for intra-and inter-day data were below 10% and 15%,respectively. |
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| Keywords: | Levamisole Residue HPLC |
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