| 硫酸链霉素细菌内毒素定量测定法的研究 |
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| 引用本文： | 冯淑霞,李金钟.硫酸链霉素细菌内毒素定量测定法的研究[J].中国兽药杂志,2006,40(3):6-8. |
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| 作者姓名： | 冯淑霞 李金钟 |
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| 作者单位： | 河北圣雪大成制药有限责任公司,河北,石家庄,051430 |
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| 摘 要： | 应用鲎试剂动态浊度法，将硫酸链霉素制备成4、2、1、0．5mg／mL溶液，定量添加标准内毒素进行干扰预试验，计算回收率，确定不干扰浓度为0．5—2mg／mL。从中选出1mg／mL为最佳浓度，然后进行3个批次硫酸链霉素正式干扰试验。在供试品中定量添加标准内毒素，其回收率分别为125．8％、126．1％、127．3％，均在50％-200％之间，说明此浓度的供试品对鲎试剂反应无干扰作用。因此，内毒素含量在0．015—1EU／mL范围内，将硫酸链霉素制备成浓度为1．0mg／mL的溶液，可用于细菌内毒素的定量检测。
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| 关 键 词： | 鲎试剂动态浊度法 细菌内毒素 硫酸链霉素 |
| 文章编号： | 1002-1280（2006）03-0006-03 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005年6月30日 |
Study on the Bacterial Endotoxin Test for Streptomycin Sulphate |
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| FENG Shu-xia,LI Jin-zhong.Study on the Bacterial Endotoxin Test for Streptomycin Sulphate[J].Chinese Journal of Veterinary Drug,2006,40(3):6-8. |
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| Authors: | FENG Shu-xia LI Jin-zhong |
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| Institution: | Hebei Shengxue Dacheng Pharmaceutical Co. , Ltd. , Shijiazhuang , Hebei 051430 |
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| Abstract: | In pre-interference experiments,the amount of endotoxin was added to 4 different concentrations of streptomycin sulphate at 4,2,1 and 0.5 mg/mL respectively.The recovery rates were calculated.It showed that the concentration 0.5~2.0 mg/mL of streptomycin sulphate did not interfere with limulus test.It was concluded that 1 mg/mL of antibiotic was the optimum concentration.On this condition,the interference experiments were conducted in three batches of streptomycin sulphate(at l mg/mL) and quantitative volumes of standards endotoxin were added, the recovery rate was 125.8%,126.1% and 127.3%,respectively.The recovery rates of endotcoxin in 3 batchs streptomycin sulphate were in the range of 50%~200%.As a conclusion,the content of endotoxin were in the range of 1,0.25,0.625 and 0.015 EU/mL,and the streptomycin sulphate solution of 1 mg/mL did not interfere with limulus test.The dynamic turbidimetric limulus test could be conveniently used for the endotoxin assay in streptomycin sulphate. |
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| Keywords: | dynamic turbidimetric limulus test streptomycin sulphate bacterial endotoxin |
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