Inhibition of potato tuber sprouting: Low levels of cytosolic pyrophosphate lead to non-sprouting tubers harvested from transgenic potato plants |
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| Authors: | Mohamad Hajirezaei Uwe Sonnewald |
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| Institution: | (1) Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstrasse 3, 06466 Gatersleben, Germany |
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| Abstract: | Summary ExpressingE. coli inorganic pyrophosphatase in transgenic plants demonstrated that long distance sucrose transport is dependent on cytosolic
pyrophosphate. It was speculated that removal of cytosolic pyrophosphate would impair sucrose utilization during storage of
potato tubers and thereby prevent tuber sprouting. To explore this hypothesisSolanum tuberosum var. Désirée plants were transformed with a chimeric PPa gene. FollowingAgrobacterium mediated transformation, metabolite and carbohydrate contents of growing and stored tubers were measured. There was a large
accumulation of soluble sugars and a decrease of starch at all developmental stages investigated. The PPase activity in PPaII
tubers was parallel with a decrease of PPa, an increase of fructose-2,6-bisphosphate and an increase of UDP-glucose. As expected
the amount of hexose-6-phosphates and glycolytic intermediates decreased. As a consequence PPaII tubers did not sprout even
after a prolonged storage period of two years. Since the energy status of PPaII tubers is unaltered inhibition of sprouting
is most likely due to reduced sucrose export and its subsequent utilization. |
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| Keywords: | pyrophosphate transgenic plants potato tuber sprouting |
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