| 不同种植地胆木根际的细菌群落结构和多样性 |
| |
| 引用本文： | 陈燕艳,徐诗涛,王德立,王军,周元元,侯祥文.不同种植地胆木根际的细菌群落结构和多样性[J].热带生物学报,2022,13(5):488-495. |
| |
| 作者姓名： | 陈燕艳 徐诗涛 王德立 王军 周元元 侯祥文 |
| |
| 作者单位： | 1.海南大学 园艺学院，海口 570228 |
| |
| 基金项目： | 海南省自然科学基金（821RC1104） |
| |
| 摘 要： | 为探明胆木根际主要细菌的群落结构和多样性，明确细菌群落的结构和多样性与土壤理化性质的相关关系，为后续开展不同种植区胆木促生根际细菌的分离筛选工作和为不同立地条件下胆木的质量评价奠定基础。采用高通量测序技术对5个种植区 15 份胆木的根际土壤样品进行分析，采用Excel、DPS和SPSS 20.0软件对数据进行多重比较和相关性分析。结果表明:15 份土壤样品平均序列长度为414～418 bp，样品DM4、DM7、DM10、DM12和DM14的平均细菌分类操作单元个数分别为1 084、1 012、1 109、870和997，获得1 223个细菌分类操作单元；从对样品的群落结构分析可知，优势细菌门为Rokubacteria、WPS-2、芽单胞菌门（Gemmatimonadetes)、浮霉菌门（Planctomycetes)、厚壁菌门（Firmicutes)、疣微菌门（Verrucomicrobia)、绿弯菌门（Chloroflexi)、放线菌门（Actinobacteria)、变形菌门（Proteobacteria)、酸杆菌门（Acidobacteria）等10个门。优势菌属为孢杆菌属（Bacillus）、酸杆菌属（Acidobacter)、链霉菌属（Streptomyces)、伯克氏菌属（Burkholderia)、Gammaproteobacteria、酸热菌属（Acidothermus)，以及未确定的菌属uncultured_bacterium_Subgroup_2 、Candidatus_Udaeobacter、Candidatus_Koribacter 和AD3；从对样品的细菌多样性分析可知，五指山的样品在物种丰度和多样性上均高于琼中县的样品；从对样品的相关性分析可知，土壤理化性质与优势菌门相关，如有机质、速效钾、有效磷和碱解氮与厚壁菌门（Firmicutes）和变形菌门（Proteobacteria）呈显著负相关，与浮霉菌门（Planctomycetes）呈显著正相关；湿度、有机质和速效钾对细菌物种丰度以及细菌多样性呈显著正相关，温度和pH值呈显著负相关。五指山和琼中种植区胆木的主要群落结构组成大体上一致，但所占丰度比不一样；细菌的群落丰度和细菌多样性具有差异，说明细菌群落丰度和细菌多样性与不同种植区相关，且五指山样品的细菌群落丰度和细菌多样性均高于琼中地区；土壤理化性质对根际细菌的主要菌门、细菌群落丰度和细菌多样性有影响。
|
| 关 键 词： | 胆木 根际土壤 细菌多样性 高通量测序 土壤理化性质 |
| 收稿时间： | 2021-12-13 |
Bacterial community structure and diversity in the rhizosphere of Nauclea officinalis in different plantations |
| |
| Institution: | 1.School of Horticulture, Hainan University, Haikou 570228, Hainan2.Hainan Branch, Institute of Medicinal Plants, Chinese Academy of Medical Sciences, Haikou, Hainan 5703113.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 570216, China |
| |
| Abstract: | An attempt was made to understand the main bacterial community structure and bacterial diversity in the rhizosphere of Nauclea officinalis to clarify the relationship between its community structure and bacterial diversity and soil physicochemical properties for further isolation and screening of rhizosphere growth-promoting bacteria in different planting areas. Fifteen rhizosphere soil samples from 5 planting areas under N. officinalis were analyzed by high-throughput sequencing technology. Multiple comparisons and correlation analysis were performed on the data using Excel, DPS and SPSS20.0 software. The results showed that the average sequence length of the 15 soil samples was 414?418 bp. The average operational taxonomic unit (OTU) numbers of samples DM4, DM7, DM10, DM12 and DM14 were 1084, 1012, 1109, 870 and 997, respectively, and 1223 bacterial taxa were obtained. The sample community structure analysis showed that there were 10 dominant bacterial phyla, such as Rokuacteria, WPS-2, Gemmatimonadetes. The dominant genera were Bacillus, Acidobacter, Streptomyces, Burkholderia, Gammaproteobacteria, Acidophilus, and the unidentified genera Candidatus, Udaeobacter, and AD3. The diversity analysis of the bacteria from the samples showed that the species abundance and diversity of the bacteria were higher in the samples form Wuzhishan city than in the samples from Qiongzhong county. The sample correlation analysis showed that soil physicochemical properties were related to dominant bacteria. For instance, organic matter, available potassium, available phosphorus and alkali-hydrolyzable nitrogen were negatively correlated significantly with Firmicutes and Proteobacteria, and positively significantly with Planctomycetes. Humidity, organic matter, available potassium were positively significantly while the temperature and pH were negatively significantly with the species abundance and diversity of the bacteria. The composition of the main community structure in the N. officinalis plantations in Wuzhishan and Qiongzhong is generally the same, but different in abundance ratios. The bacterial community abundance and bacterial diversity were different in these two areas, indicating that the bacterial community abundance and bacterial diversity are different in different planting areas. Moreover, the bacterial community abundance and bacterial diversity in the samples from Wuzhishan were higher than those from Qiongzhong. The soil physicochemical properties had an impact on the main phyla, bacterial community abundance and bacterial diversity of rhizosphere bacteria. |
| |
| Keywords: | |
|
| 点击此处可从《热带生物学报》浏览原始摘要信息 |
| 点击此处可从《热带生物学报》下载免费的PDF全文 |
|
