| 牛奶中不同酪蛋白遗传变异体及其分型鉴定研究 |
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| 引用本文： | 谭莲英,夏忠悦,钱成林,董成虎,宋艳梅,范光彩.牛奶中不同酪蛋白遗传变异体及其分型鉴定研究[J].中国乳业,2022,0(12):120-124. |
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| 作者姓名： | 谭莲英 夏忠悦 钱成林 董成虎 宋艳梅 范光彩 |
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| 作者单位： | 1 新希望乳业股份有限公司,四川成都 610011; 2 乳品营养与功能四川省重点实验室,四川成都 611730; 3 夏忠悦劳模和工匠人才创新工作室,四川成都 611730 |
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| 基金项目： | 乳制品及牛肉制品精深加工关键技术研究与产业化应用示范（2020YFN0153） |
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| 摘 要： | 牛奶中存在不同种类的蛋白质,且不同基因型遗传变异体牛奶蛋白质的构型和功效存在差异。酪蛋白分型鉴定方法有基因鉴定、毛细管电泳分离鉴定、高效液相色谱分离鉴定等。本文对比介绍了不同构型κ-酪蛋白和β-酪蛋白的分型鉴定方法,重点验证了高效液相色分离谱鉴定方法的精密度、回收率,同时将该方法应用于生乳和乳制品酪蛋白分型检测鉴定,区分不同牧场牛群的遗传变异体类型和比例。通过该方法可指导牧场快速分群饲养奶牛,生产不同酪蛋白类型的生乳,并加工不同类型的高品质乳制品。
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| 关 键 词： | 牛奶 酪蛋白 基因亚型 分型鉴定 |
Genetic Variants of Casein in Milk and Their Identification |
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| TAN Lianying,XIA Zhongyue,QIAN Chenglin,DONGChenghu,SONG Yanmei,FAN Guangcai.Genetic Variants of Casein in Milk and Their Identification[J].China Dairy,2022,0(12):120-124. |
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| Authors: | TAN Lianying XIA Zhongyue QIAN Chenglin DONGChenghu SONG Yanmei FAN Guangcai |
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| Institution: | 1New Hope Dairy Co.,LTD.,Chengdu Sichuan 610011; 2Dairy Nutrition and Function,Key Laboratory of Sichuan Province,Chengdu Sichuan 611730; 3Xia Zhongyue Labor Model and Artisan Talent Innovation Studio,Chengdu Sichuan 611730 |
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| Abstract: | There are different kinds of proteins in milk,and the configuration and efficacy of milk proteins of different genetic variants are different. Casein typing identification methods include gene identification,capillary electrophoresis identification,high performance liquid chromatography identification,etc. In this paper,the typing and identification methods of K-casein and β-casein with different configurations were compared,and the precision and recovery rate of the HPLC chromatographic identification method were mainly verified. Meanwhile,the method was applied to the typing and identification of raw milk and dairy casein to distinguish the types and proportions of genetic variants in cattle in different pastures. This method can guide the pasture to breed dairy cows quickly,produce raw milk with different casein types,and process different types of high quality dairy products. |
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| Keywords: | milk casein gene subtype classification identification |
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