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| 侵染云南鸡蛋果的病毒种类鉴定及CMV cp 基因序列分析 | |
| 引用本文： | 柳勤海,兰平秀,吕霞,李凡,谭冠林.侵染云南鸡蛋果的病毒种类鉴定及CMV cp 基因序列分析[J].植物病理学报,2022,52(6):1021-1024. |
| 作者姓名： | 柳勤海 兰平秀 吕霞 李凡 谭冠林 |
| 作者单位： | 云南农业大学植物保护学院,昆明 650201; 云南农业大学现代教育技术中心,昆明 650201; 云南农业大学,云南省滇台特色农业产业化工程研究中心,昆明 650201 |
| 基金项目： | 国家自然科学基金(31660039); 云南省院士专家工作站(202005AF150040) |
| 摘 要： | |
| 收稿时间： | 2021-09-21 |
Identification of the viruses infecting Passiflora edulis in Yunnan and sequence analysis of the cp gene of CMV infecting P. edulis |
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| LIU Qinhai,LAN Pingxiu,Lü Xia,LI Fan,TAN Guanlin.Identification of the viruses infecting Passiflora edulis in Yunnan and sequence analysis of the cp gene of CMV infecting P. edulis [J].Acta Phytopathologica Sinica,2022,52(6):1021-1024. | |
| Authors: | LIU Qinhai LAN Pingxiu Lü Xia LI Fan TAN Guanlin |
| Institution: | College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China; Modern Education Technology Center, Yunnan Agricultural University, Kunming 650201, China; Yunnan-Taiwan Engineering Research Center for Characteristic Agriculture Industrialization of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China |
| Abstract: | Viral diseased Passiflora edulis samples showing mosaic, shrinking, chlorosis, and malformation were collected from Yuxi, Wenshan, Dehong, and Xishuangbanna in Yunnan Province. Forty diseased samples were detected by RT-PCR/PCR using degenerate primers of members in genera of Umbravirus, Tobamovirus, Luteovirus, Orthotospovirus, Begomovirus, Badnavirus, Potyvirus and specific primers of cucumber mosaic virus (CMV). Results showed that CMV, telosma mosaic virus (TeMV) and papaya leaf curl Guangdong virus (PaLCuGdV) were detected in the diseased P. edulis samples. CMV showed the highest detection rate of 27.5 %, while TeMV and PaLCuGdV had the rates of 20.0% and 7.5% respectively among the P. edulis samples. Mixed infections of PaLCuGdV+CMV, PaLCuGdV+TeMV and CMV+TeMV were also found in these samples. The 657 nucleotide (nt) CMV full-length coat protein (CP) gene was cloned and sequenced from two diseased samples in Yuxi (YYXi-JDG, Acc. No. MW495062) and Xishuangbanna (YXSBN-JDG, Acc. No. MW495063), respectively. These two CMV isolates shared 97.3% identity of nt sequence with each other, while had 76.9%-97.7% and 77.0%-98.2% nt identity with other CMV isolates, respectively. Phylogenetic tree showed that the two CMV isolates from P. edulis belonged to CMV subgroup Ⅰ, and no obvious geographic differentiation and host specificity relationship was found among the selected 33 CMV isolates worldwide. |
| Keywords: | Passiflora edulis virus disease cucumber mosaic virus telosma mosaic virus papaya leaf curl Guangdong virus phylogenetic analysis |
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