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| 山茶炭疽菌CcSnf1基因参与调控山茶炭疽菌的生长发育和致病力 | |
| 引用本文： | 徐小文,王义勋,张子一,沈蜜.山茶炭疽菌CcSnf1基因参与调控山茶炭疽菌的生长发育和致病力[J].植物病理学报,2022,52(6):927-939. |
| 作者姓名： | 徐小文 王义勋 张子一 沈蜜 |
| 作者单位： | 湖北省林业科学研究院,武汉 430075; 林木种质改良与资源综合利用湖北省重点实验室,大别山特色资源开发湖北省协同创新中心,黄冈师范学院,黄冈 438000 |
| 基金项目： | 经济林木种质改良与资源综合利用湖北省重点实验室大别山特色资源开发湖北省协同创新中心开放基金(201930703) |
| 摘 要： | 油茶炭疽病是油茶上的重要病害,山茶炭疽菌(Colletotrichum camelliae)是该病害的优势病原菌。本研究以山茶炭疽菌中丝氨酸/苏氨酸类蛋白激酶SNF1蛋白复合体的核心组分CcSnf1为研究对象,研究其在山茶炭疽菌的营养生长、产孢量、孢子萌发、附着胞形成、对环境胁迫因子的响应、对不同碳源的利用、致病力等方面的生物学功能,为油茶炭疽病的防控提供理论依据。研究结果表明,山茶炭疽菌CcSnf1基因编码的蛋白为719个氨基酸,含有一个蛋白激酶催化结构域、一个泛素相关结构域和一个腺苷酸感应器结构域。CcSnf1是果生炭疽菌CfSnf1和胶孢炭疽菌CgSnf1的高度同源物。该基因的敲除体菌丝生长减慢,分生孢子产量显著降低,对不同碳源、细胞壁完整性因子和渗透压胁迫因子有不同程度的敏感性。侵染早期阶段的孢子萌发率和附着胞形成率显著受影响,随后CcSnf1的敲除体对油茶果实和叶片的致病力明显减弱。本研究表明CcSnf1在山茶炭疽菌的菌丝生长、产孢、胁迫因子响应、对特定碳源的利用和致病力具有重要的作用。 |
| 关 键 词： | 油茶 山茶炭疽菌 Snf1 致病力 |
| 收稿时间： | 2021-12-21 |
CcSnf1 is involved in regulating development and pathogenecity in Colletotrichum camelliae |
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| XU Xiaowen,WANG Yixun,ZHANG Ziyi,SHEN Mi.CcSnf1 is involved in regulating development and pathogenecity in Colletotrichum camelliae[J].Acta Phytopathologica Sinica,2022,52(6):927-939. | |
| Authors: | XU Xiaowen WANG Yixun ZHANG Ziyi SHEN Mi |
| Institution: | Hubei Academy of Forestry, Wuhan 430075,China; Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang 438000, China |
| Abstract: | Anthracnose on Camellia oleifera is an important disease of Camellia in China, and the Colletotrichum camelliae is the dominant pathogen of the disease. In this study, CcSnf1, the core component of serine / threonine protein kinase SNF1 protein complex in Co. camellia, was used to study its biological functions in vegetative growth, conidiation, conidial germination, appressorium formation, response to environmental stress factors, utilization of different carbon sources and pathogenicity. The results showed that CcSnf1 encode a proteinwith 719 amino acids and with a protein kinase catalytic domain, a ubiquitin-associated domain and a adenylate sensor domain. CcSnf1 is a high homologue of Co. fructicola CfSnf1 and Co. gloeosporoides CgSnf1. Deletion of CcSnf1 showed reduced mycelial growth and conidiation. The ΔCcSnf1 mutants also exhibited different sensitivity to various carbon sources, cell wall stressor and osmotic stress factors. The pre-penetration stage including conidial germination and appressorium formation of the ΔCcSnf1 was largely impaired, and the pathogenicity of the ΔCcSnf1 on C. oleifera fruits and leaves was significantly decreased. Our results suggest that CcSnf1 is critical for mycelial growth, conidiation, stress responses, utilization of specific carbon sources and pathogenicity of Co. camelliae. The results of the study will provide a theoretical basis for the prevention and control of anthracnose in oil tea. |
| Keywords: | Camellia oleifera Colletotrichum camelliae Snf1 pathogenicity |
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