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| 侵染白附子的芋花叶病毒分子变异研究 | |
| 引用本文： | 秦艳红,高素霞,刘玉霞,文艺,杨瑾,李雪梦,王凤丽,戚文平,康宇静,王飞,鲁传涛.侵染白附子的芋花叶病毒分子变异研究[J].植物病理学报,2022,52(6):881-890. |
| 作者姓名： | 秦艳红 高素霞 刘玉霞 文艺 杨瑾 李雪梦 王凤丽 戚文平 康宇静 王飞 鲁传涛 |
| 作者单位： | 河南省农业科学院植物保护研究所,河南省农作物病虫害防治重点实验室,农业部华北南部作物有害生物综合治理重点实验室,郑州 450002; 开封市农林科学研究院,开封 475004 |
| 基金项目： | 国家中药材产业技术体系(CARS-B21);河南省中药材产业技术体系(Z2019-13-01);河南省农业科学院新兴学科发展专项(2020XK05、2021XK02);河南省农业科学院基本科研业务费项目(2021ZC47) |
| 摘 要： | 为明确侵染白附子的芋花叶病毒(dasheen mosaic virus,DsMV)的分子变异情况,对51个DsMV白附子分离物(DsMV-BF)的外壳蛋白(Coat Protein,CP)基因和3个分离物的近全长基因组序列进行了克隆和测定,DsMV-BF的CP基因大小有855个和942个核苷酸两种类型,51个白附子分离物之间CP基因的核苷酸和氨基酸一致率分别为88.3%~100%和91.9%~100%,BF8、BF30和BF38分离物之间多聚蛋白的核苷酸和氨基酸序列一致率分别为82.9%~95.9%和90.7%~95.9%,与GenBank中其他分离物之间多聚蛋白的核苷酸和氨基酸序列一致率分别为76.9%~99.4%和85.6%~99.0%;P1基因的分子变异较大,P1基因大小有987个和990 个核苷酸两种类型;CP基因核苷酸序列系统进化树分析结果表明,侵染白附子的DsMV分离物可分为两个亚组;重组分析结果表明BF8和BF30分离物各检测到1个重组事件,BF38检测到2个重组事件。 |
| 关 键 词： | 芋花叶病毒 分子变异 重组分析 |
| 收稿时间： | 2021-10-21 |
Molecular variability of the dasheen mosaic virus infecting Typhonium giganteum Engl. |
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| QIN Yanhong,GAO Suxia,LIU Yuxia,WEN Yi,YANG Jin,LI Xuemeng,WANG Fengli,QI Wenping,KANG Yujing,WANG fei,LU Chuantao.Molecular variability of the dasheen mosaic virus infecting Typhonium giganteum Engl.[J].Acta Phytopathologica Sinica,2022,52(6):881-890. | |
| Authors: | QIN Yanhong GAO Suxia LIU Yuxia WEN Yi YANG Jin LI Xuemeng WANG Fengli QI Wenping KANG Yujing WANG fei LU Chuantao |
| Institution: | Henan Key Laboratory of Crop Pest Control, Integrated Pest Management Key Laboratory in Southern part of North China for Ministry of Agriculture, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; Kaifeng Academy of Agriculture and Forestry, Kaifeng 475004, China |
| Abstract: | To elucidate the molecular diversity of dasheen mosaic virus (DsMV) infecting T. giganteum Engl, the coat protein (CP) genes of fifty-one DsMV-BF isolates and near-complete genomic sequences of three isolates were cloned and sequenced. The CP gene of DsMV-BF isolates had the length of 855 bp and 942 bp. Pairwise comparisons of CP gene nucleotide (nt) and amino acid (aa) sequence showed that 51 DsMV-BF isolates had 88.3%-100% nt and 91.9%-100% aa sequence identity with each other. Pairwise comparisons of polyprotein gene sequences showed that BF8, BF30 and BF38 isolates had 82.9%-95.9% nt and 90.7%-95.9% aa sequence identity with each other. Whereas, they displayed 76.9%-99.4% nt and 85.6%-99.0% aa identity with other isolates in the GenBank. P1 gene having lengths of 987 bp or 990 bp, exhibited the highest genetic variability. Phylogenetic tree analysis based on CP gene indicated that DsMV-BF isolates were divided into two subgroups. Recombination analysis showed that BF8 and BF30 isolates were associated with one potential recombination event, respectively. Furthermore, BF38 isolate was detected to have two potential recombination events. |
| Keywords: | dasheen mosaic virus molecular variability recombination analysis |
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