| 结球甘蓝BoCBF1与BoCBF2基因的CAPS标记及其对耐寒性的影响 |
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| 引用本文： | 靳哲,张扬勇,方智远,刘玉梅,杨丽梅,庄木,刘基生,孙培田.结球甘蓝BoCBF1与BoCBF2基因的CAPS标记及其对耐寒性的影响[J].中国蔬菜,2012,1(14):23-30. |
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| 作者姓名： | 靳哲 张扬勇 方智远 刘玉梅 杨丽梅 庄木 刘基生 孙培田 |
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| 作者单位： | 中国农业科学院蔬菜花卉研究所，北京 100081 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项;北京市科委重大项目(D121100003412001);国家“十一五”科技支撑计划项目(2009BADB8B03);农业部大宗蔬菜产业技术体系项目(CARS-25),农业部园艺作物生物学与种质创制重点实验室项目 |
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| 摘 要： | 以两份具有不同耐寒性的甘蓝自交系341和21-3为试材，根据已有拟南芥CBF1基因序列及甘蓝基因组测序拼接出的Scaffold信息，获得了甘蓝BoCBF1和BoCBF2基因编码区的全长序列。经PCR产物直接测序发现BoCBF1基因在自交系341和21-3间的变异表现为4个单核苷酸多态性（SNP），而BoCBF2基因则表现为16个SNP及1个18 bp碱基的插入缺失（InDel）。为简便快捷地鉴定两个自交系间BoCBF1和BoCBF2基因序列的变异，开发了BoCBF1和BoCBF2基因的CAPS标记，分别命名为BoCBF1-TaqαⅠ和BoCBF2-BstUⅠ。利用这两个CAPS标记对两个自交系、F1、F2群体的基因型与耐寒性进行相关性分析，发现这两个基因的变异与材料的耐寒性极显著相关，可用该标记进行甘蓝耐寒育种的分子标记辅助选择。
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| 关 键 词： | 结球甘蓝 BoCBF1基因 BoCBF2基因 CAPS标记 耐寒性 |
CAPS Markers of BoCBF1 and BoCBF2 Genes in Cabbage and Their Contribution to Cold-tolerance |
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| JIN Zhe,ZHANG Yang-yong,FANG Zhi-yuan,LIU Yu-mei,YANG Li-mei,ZHUANG Mu,LIU Ji-sheng,SUN Pei-tian.CAPS Markers of BoCBF1 and BoCBF2 Genes in Cabbage and Their Contribution to Cold-tolerance[J].China Vegetables,2012,1(14):23-30. |
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| Authors: | JIN Zhe ZHANG Yang-yong FANG Zhi-yuan LIU Yu-mei YANG Li-mei ZHUANG Mu LIU Ji-sheng SUN Pei-tian |
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| Institution: | Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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| Abstract: | Two cabbage（Brassica oleracea var. capitata L.）inbred lines differing in cold-tolerance were used to analyze the effect of CBF gene variation on cold-tolerance．Based on Arabidopsis CBF1 gene sequence and the scaffold information of cabbage genome，the full-length coding domain sequence of cabbage BoCBF1 and BoCBF2 genes were obtained．PCR products were sequenced directly to discover 4 single nucleotide polymorphisms （SNP）between the inbred lines 341 and 21-3 for BoCBF1 gene and 16 SNP and one insertion-deletion （InDel）for BoCBF2 gene．In order to rapidly and reliably detect the sequence variation of BoCBF1 and BoCBF2 genes，2 CAPS markers were successfully converted and named as BoCBF1-TaqαⅠ，BoCBF2-BstUⅠ．The CAPS markers were used to screen inbred lines 341，21-3 and their F1，F2 population．The analysis showed that the variation of 2 genes very significantly correlated with cold-tolerance，so the CAPS markers can be used for marker-assisted selection（MAS）in cabbage cold-tolerance breeding． |
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| Keywords: | BoCBF1 gene BoCBF2 gene CAPS marker Cold-tolerance |
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